Soil microbial communities are among the richest sources of biodiversity on Earth. However, very little is known about the roles of individual microbial populations living in soil and how they affect important processes such as soil fertility and carbon cycling. This proposal investigates free-living Bradyrhizobium, an abundant and widespread soil bacterium, and will provide mechanistic… [Read More]

Collected at the Southern Ocean Time Series, transcriptomes and manipulative experiments will establish virus and microbial processes that constrain the carbon cycle and system biogeochemistry (major DOE mission areas). Interactions on sinking particles, the specificity of virus-host relationships and trace metal effects will be examined. The cumulative data set will allow for the development of… [Read More]

Roots and stomata are plants’ primary entry and exit points for water. Therefore, they are key targets for manipulation to improve bioenergy crop productivity, resilience and sustainability by reducing the risk of crop losses due to inadequate water supply. The number of pores, called stomata, on the leaf surface influences how much water the crop… [Read More]

Freshwater lakes contain diverse and dynamic microbial communities that play key roles in maintaining water quality, cycling nutrients, and regulating carbon uptake and storage. They are important hotspots for biogeochemical cycling in terrestrial landscapes. This project aims to use hundreds of samples collected over time in two very different lakes to understand how these communities… [Read More]

The analysis of gene expression allows scientists to generate hypotheses important for the understanding of gene function. Proteins that participate in similar functions, or form larger protein complexes, are often encoded by genes that share similar expression profiles. This type of analysis has been limited to “steady-state” mRNA levels, that is the combined output of… [Read More]

The proposal calls for sequencing accession (genetically unique plant samples from a particular geographic location) representatives of the Saccharum genus and key sugarcane cultivars to elucidate their genome structure, origin and phylogenetic relationships. This information will help optimize breeding and base-broadening programs and builds off the ongoing sugarcane genome proposal. Proposer: Angelique D’Hont, CIRAD (France)… [Read More]

Plant biomass degradation by fungi is a key step of the carbon cycle on earth, but carbon sequestration is also mediated by mutualistic fungi through symbiosis with plants. Indeed plant material is the most abundant repository of organic carbon and fungi are the primary decomposers of dead or alive plant cell walls. To do so,… [Read More]

Genomes of important model and many industrially important fungi are still incomplete. The goals of this project are: (a) to finish and annotate the genomes of ~25 strains; and, (b) to carry out functional analyses by novel CRISPR/Cas9 approaches. The next quantum leap in biology will come by integrating all levels of genome, transcriptome, proteome,… [Read More]