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    A vertical tree stump outdoors with about a dozen shiitake mushrooms sprouting from its surface.
    Tracing the Evolution of Shiitake Mushrooms
    Understanding Lentinula genomes and their evolution could provide strategies for converting plant waste into sugars for biofuel production. Additionally, these fungi play a role in the global carbon cycle.

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    Soil Virus Offers Insight into Maintaining Microorganisms
    Through a collaborative effort, researchers have identified a protein in soil viruses that may promote soil health.

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    Data yielded from RIViT-seq increased the number of sigma factor-gene pairs confirmed in Streptomyces coelicolor from 209 to 399. Here, grey arrows denote previously known regulation and red arrows are regulation identified by RIViT-seq; orange nodes mark sigma factors while gray nodes mark other genes. (Otani, H., Mouncey, N.J. Nat Commun 13, 3502 (2022). https://doi.org/10.1038/s41467-022-31191-w)
    Streamlining Regulon Identification in Bacteria
    Regulons are a group of genes that can be turned on or off by the same regulatory protein. RIViT-seq technology could speed up associating transcription factors with their target genes.

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    The switchgrass diversity panel growing at the Kellogg Biological Station in Michigan. (David Lowry)
    Mapping Switchgrass Traits with Common Gardens
    The combination of field data and genetic information has allowed researchers to associate climate adaptations with switchgrass biology.

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    Artist rendering of genome standards being applied to deciphering the extensive diversity of viruses. (Illustration by Leah Pantea)
    Expanding Metagenomics to Capture Viral Diversity
    Along with highlighting the viruses in a given sample, metagenomics shed light on another key aspect of viruses in the environment — their sheer genetic diversity.

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    Photograph of a stream of diatoms beneath Arctic sea ice.
    Polar Phytoplankton Need Zinc to Cope with the Cold
    As part of a long-term collaboration with the JGI Algal Program, researchers studying function and activity of phytoplankton genes in polar waters have found that these algae rely on dissolved zinc to photosynthesize.

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    Silver Age of GOLD Introduces New Features
    The Genomes OnLine Database makes curated microbiome metadata that follows community standards freely available and enables large-scale comparative genomics analysis initiatives.

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    Graphical overview of the RNA Virus MetaTranscriptomes Project. (Courtesy of Simon Roux)
    A Better Way to Find RNA Virus Needles in the Proverbial Database Haystacks
    Researchers combed through more than 5,000 data sets of RNA sequences generated from diverse environmental samples around the world, resulting in a five-fold increase of RNA virus diversity.

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    HPCwire Editor's Choice Award (logo crop) for Best Use of HPC in the Life Sciences
    JGI Part of Berkeley Lab Team Awarded Best Use of HPC in Life Sciences
    The HPCwire Editors Choice Award for Best Use of HPC in Life Sciences went to the Berkeley Lab team comprised of JGI and ExaBiome Project team, supported by the DOE Exascale Computing Project for MetaHipMer, an end-to-end genome assembler that supports “an unprecedented assembly of environmental microbiomes.”

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    Digital ID card with six headshots reads: Congratulations to our 2022 Function Genomics recipients!
    Final Round of 2022 CSP Functional Genomics Awardees
    Meet the final six researchers whose proposals were selected for the 2022 Community Science Program Functional Genomics call.

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    CSP New Investigators FY23 R1
    JGI Announces First Round of 2023 New Investigator Awardees
    Twice each year we look for novel research projects aligned with DOE missions and from PIs who have not led any previously-accepted proposals through the CSP New Investigator call.

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    screencap from Amundson and Wilkins subsurface microbiome video
    Digging into Microbial Ecosystems Deep Underground
    JGI users and microbiome researchers at Colorado State University have many questions about the microbial communities deep underground, including the role viral infection may play in other natural ecosystems.

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    2022 JGI-UC Merced interns (Thor Swift/Berkeley Lab)
    Exploring Possibilities: 2022 JGI-UC Merced Interns
    The 2022 UC Merced intern cohort share how their summer internship experiences have influenced their careers in science.

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    Using Team Science to Build Communities Around Data
    As the data portals grow and evolve, the research communities further expand around them. But with two projects, communities are forming to generate high quality genomes to benefit researchers.

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    Cow Rumen and the Early Days of Metagenomics
    Tracing a cow rumen dataset from the lab to material for a hands-on undergraduate research course at CSU-San Marcos that has since expanded into three other universities.

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September 22, 2017

Comparative and Population Genomics of Xylariaceae

Morphological diversity of Xylariaceous endophytes. (J.M. U'Ren)Despite evidence that endophytes play a critical role in plant-microbe interactions, they are poorly represented in genome databases. Leveraging our unique culture collection, the team proposes a genomic survey of the Xylariaceae, one of the largest and most diverse families of fungi made up of endophytic, pathogenic, and saprotrophic (including wood degrading) species. Our project… [Read More]

September 22, 2017

How Nectar Yeasts Scavenge Nitrogen

Nectar yeast, Metschnikowia gruessii. (Manpreet Dhami, Tadashi Fukami, and Lydia-Marie Joubert)The species of yeasts that colonize floral nectar face two major challenges: high osmotic pressure caused by excessive carbon supply and strong resource competition caused by low nitrogen availability. This project is aimed at identifying the genes and pathways that enable nectar yeasts to grow in the carbon- and nitrogen-stressed environment. Nectar yeasts have presumably… [Read More]

September 22, 2017

Open Green Genomes Initiative

The Open Green Genomes Initiative will generate high-quality genome assemblies and annotations for 35 species representing all major evolutionary lineages in the land plant tree of life. This work will greatly improve comparative analyses of the genes, regulatory networks and metabolic pathways influencing plant growth, responses to environmental stress, and production of valuable plant products…. [Read More]

September 22, 2017

Gene Atlases of Grass-Microbe Interactions

Virus-infected Brachypodium (Courtesy of Kranthi Mandadi)Grasses comprise key food crops, as well as bioenergy feedstock. However, their productivity and biomass is greatly hindered by viral, fungal, and bacterial infections, which cause yield losses of up to 60 percent. This proposal seeks to build comprehensive gene atlas maps for diverse bioenergy grass-microbe interactions, including pathogenic and beneficial interactions in two grass… [Read More]

September 22, 2017

Shaping the Brachypodium Polyploid Model

Polyploid plants are often larger than their diploid progenitors and can be more stress tolerant. Some of the world’s most important crops are polyploid. This project uses three small grasses with compact genomes and traits that make them easy to manipulate in the laboratory. Brachypodium hybridum is an allotetraploid formed by interspecifc hybridization between the… [Read More]

September 22, 2017

Improving Crop Productivity Strategies

The sustainable production of biofuels from energy crops could greatly benefit from strategies that increase crop productivity in existing agricultural lands. Application of rare earth enzymes (REE) increases crop yields but the molecular mechanisms by which REE increase plant productivity are not well understood. As REE affect the metabolism of methylotrophs, a predominant member of… [Read More]

September 22, 2017

Impact of Plant Cell Wall Modification

ORNL researchers are testing the hypothesis that genetic modification of plant cell wall has cascading and quantifiable impacts on its secondary metabolome and the associated functional microbiome. The hypothesis is based on the team’s recent finding that modification of a plant cell wall pathway gene, PdKOR, an endoglucanase, in Populus can impact its ability to… [Read More]

September 22, 2017

Metabolic Diversity of Sorghum and Maize

Leveraging genetic and biochemical approaches with JGI contributions, researchers aim to better understand the metabolic diversity of sorghum and maize, how that metabolic diversity is generated, and how specific metabolites and pathways mediate plant interactions with the microbial community. Together these resources and knowledge are anticipated to guide development of next generation Poaceous fuel crops… [Read More]

September 22, 2017

Phenomics of the Model Alga Chlamydomonas reinhardtii

Heat stress impairs plant growth and reduces crop yield. In nature, temperature and day length are linked together; longer days tend to have higher temperatures than shorter days. Little is known about how photosynthetic organisms regulate and integrate their responses to high temperature and day length to optimize growth. The team will use green alga… [Read More]

September 22, 2017

Bacteria and Fungi in Native Prairies

Despite the persistent presence of potentially harmful fungi and bacteria, America’s native prairies are healthy, thriving communities of perennial herbaceous plants. How do these communities maintain a balance with these microbes? Bacteria and fungi found in native prairie soil, are mutually antagonist and thus may keep each other in check. Each produces antibiotic compounds when… [Read More]
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