About The CSP Annual Call
The CSP Annual Call is focused on large-scale sequence-based genomic science projects that address questions of relevance to DOE missions in energy security and sustainability and global biogeochemistry. While applications will be accepted that address any aspect of these mission areas, priority for this call will be given to projects that address the specific areas of special emphasis that are listed in the call, and exploit the diversity of JGI technical and analytical capabilities.
For the CSP annual call, a Letter of Intent (LOI) is required to submit a proposal to JGI. The letter of intent must be submitted using our web-based form. Submitters whose LOIs/proposals are approved will receive further instructions via email. Human or animal disease-causing organisms will NOT be considered under this Program. Only plant pathogens of relevance to bioenergy-related crops or plants will be considered. All proposals will be reviewed for scientific merit, DOE relevance, proposed data usage by the applicant/community, and track record of the applicant. Proposers are encouraged to review JGI’s sample requirements; samples that are not available in a timely manner, or are of insufficient quality or quantity, will delay or potentially defer approval for sequencing.
View the list of accepted proposals from previous calls.
For questions about the appropriateness of projects, program specifics or application process, please contact Susannah Tringe.
The DOE JGI’s Community Science Program (CSP) is now accepting Letters of Intent for large-scale sequence-based genomic science projects that address questions of relevance to DOE Biological and Environmental Research (BER) missions in sustainable biofuel production, global carbon cycling, and biogeochemistry. While applications will be accepted that address any aspect of the DOE mission areas, special consideration will be given to projects that address the following areas of emphasis and exploit the diversity of DOE JGI capabilities.
I. Plant Functional Genomics and Microbiomes:
The DOE JGI has produced several “flagship plant genomes” including sorghum, Brachypodium, and Physcomitrella and is developing genomes for emerging flagship plant species including switchgrass and Miscanthus. These species are of special interest as potential biofuel feedstocks or as comparators that provide insight into feedstock evolution and phenotype, and projects that directly relate to these genomes are encouraged. For all plant proposals, priority will be given to multi-organism proposals that 1) seek to compare among plants and/or analyze plant-microbiome interactions, and/or 2) are of a large, collaborative nature with multiple participating investigators. Projects of interest may fall into one of the following four categories:
a) Gene Atlas and ENCODE-like projects – The DOE JGI is currently committed to sequencing flagship plant transcriptomes under a variety of experimental conditions for different plant tissues and developmental stages. New proposals are encouraged that expand the experimental conditions or plants to be studied and extend functional studies beyond straightforward transcriptomics. This includes proposals aimed at the generation of genome-wide annotation of gene regulatory sequences, similar to the NIH-funded ENCODE encyclopedia of DNA elements in the human genome (See core capabilities below for available assays).
b) Large-scale germplasm resequencing – We invite germplasm resequencing projects aimed at 1) understanding natural population structure of the genus/species, 2) creating a foundation for large scale GWAS projects for gene discovery, or 3) developing pan and core genomes to determine a complete picture of gene content within the genus/species. Studies must target mission relevant plants with existing high quality genomes.
c) High quality or comparative grade de novo genomes – We invite proposals for whole genome sequencing of species that can be used for comparative genomics studies with the DOE JGI flagship species. Proposals should justify the relevance as comparators, including enabling the identification of conserved and selected DNA elements and increasing our ability to infer gene function across plant phylogenetic space. Requests for high quality genomes must clearly indicate the size of the user community and what BER mission related science will be enabled above and beyond what could be accomplished with a comparative grade draft.
d) Plant microbiomes – We encourage projects to study the microbiomes of BER mission relevant plants. Proposals aimed at characterizing secondary metabolite biosynthetic pathways in plants and/or associated microbes are specifically encouraged, as are hypothesis-driven projects deciphering functional and phylogenetic changes of natural or synthetic communities upon manipulation of the host and/or host environment. We encourage proposal submitters to consider using KBase to model these interactions.
II. Inter-organismal interactions:
A key focus for DOE JGI is understanding the mutualistic, competitive or antagonistic interactions among microorganisms, macroorganisms, and viruses. Projects that could address this focus include:
a) Investigation of the genomic basis of microbial mutualism and microbe-microbe interactions in stable model communities, e.g. enrichment cultures or synthetic communities.
b) Functional and chemical characterization of secondary metabolites that are involved in inter-organism interactions, leveraging sequencing, synthetic biology, transcriptomics, and metabolomics.
c) Function-driven single-cell genomics and metagenomics, e.g. sequencing of stable isotope-labeled DNA or selectively sorted single cells to assign functional roles to populations within communities.
d) Genomic investigation of viral evolution and host specificity.
III. Microbes and communities involved in elemental cycling in terrestrial and coastal environments
Bacteria, archaea, fungi and algae are important consumers and producers of greenhouse gases in the environment. While a nascent understanding of nutrient cycling in marine environments exists, our understanding of these complex processes in natural terrestrial environments has lagged behind. Proposals are encouraged that will provide insight into microbial activities controlling global cycles of carbon, nitrogen, phosphorus, and sulfur from a broad range of terrestrial and coastal environments (including terrestrial-aquatic interfaces such as peat bogs, marshes, and hyporheic zones). In addition, developing multi-omics datasets to enable modeling of regulatory and metabolic processing of these elements in model microbes and microbial systems is encouraged
IV. Algal genomics
Algae are important primary producers with tremendous diversity, long evolutionary history, and huge potential for DOE science and applications. Significant and rapid advances in the fundamental knowledge of algal biology, the entire biomass-to-bioenergy supply chain, and algal cultivation strategies are dependent on ecological, genetic and biochemical information which is currently lacking. Proposals are encouraged that will expand genomic knowledge across algal diversity, that will build fundamental knowledge of algal metabolism and physiology, and which will provide insights into algal associations with other microbes.
CSP projects are expected to generate publicly available data that will answer important questions relevant to the target organism or environment as well as providing the substrate for broader use by the DOE research community. CSP projects have historically provided a means for user communities to assemble and interact in collaborative ways. Proposals are encouraged that involve some or all of the following features: 1) a scale and complexity that exceeds the capacity of a single lab, 2) engaging a large group of collaborators, 3) requiring DOE JGI capabilities that reach beyond genome sequencing, 4) generating data of high value to the scientific community, and 5) plans to analyze and distribute data and results through the DOE Systems Biology Knowledgebase (KBase).
All proposals may request up to 2.5 Tbp of sequence data. For multi-PI projects generating data of broad utility to the scientific community, requests of up to 10 Tbp will be considered. Even larger Tbp totals (up to 40 Tbp) will be considered for shotgun Illumina DNA sequencing only (i.e. plant resequencing and metagenome sequencing), but such proposals will be evaluated separately with the anticipation that only 1 or 2 would be approved.
Requests for Pacific Biosciences long read sequencing are capped at 25 Gbp, while requests of up to 50-100 Gbp will be considered for multi-PI projects of high value to the scientific community.
The DOE JGI provides extensive data analysis pipelines. Applicants should present a plan for all data analysis that may be required beyond these standard pipelines. Users are encouraged to consider how KBase may be used or extended to meet these needs.
DOE JGI Capabilities
The DOE JGI employs an evolving suite of sequencing platforms, currently comprised of short read Illumina as well as single molecule long-read Pacific Biosciences technology. The capabilities available for this call are listed below. While individual proposals may draw from one or more of these capabilities as needed to fulfill project goals, within the overall cap, the final scope is ultimately at the discretion of the DOE JGI. Successful projects frequently utilize a combination of capabilities:
Core Capabilities Include:
- De novo sequencing of fungal, algal, bacterial, archaeal, viral and plant genomes
- Resequencing and target-enriched resequencing for variation detection
- Microbial community shotgun DNA/RNA sequencing (not amplicon sequencing, which is no longer offered)
- Whole genome DNA methylation analysis
- Comprehensive transcriptome analysis including coding transcript annotation, non-coding RNA (both small and long ncRNA) characterization and expression profiling
- Fluorescence activated cell sorting for targeted metagenomics and single-cell genomics
- DNA/gene synthesis linked to sequence data generation, including codon optimization, refactoring, and assembly of biosynthetic pathways into appropriate vector systems for expression in heterologous hosts. (Use of this capability is encouraged, but synthesis-only projects should be directed to the call for stand-alone DNA synthesis proposals)
- Mass spectrometry-based metabolomics analysis of primary and secondary metabolites from plants and microorganisms (Use of this capability is encouraged, but metabolomics-only projects will not be considered at this time)
- Analysis pipelines for the datasets above
The DOE JGI also has limited capacity for the following developing capabilities, when tightly linked to sequencing or DNA synthesis:
- Custom analysis of DOE JGI datasets
- Chromatin analysis including mapping of histone modifications by Chromatin Immunoprecipitation (ChIP-seq), and open chromatin by Assay for Transposase-Accessible Chromatin (ATAC-seq).
- In vitro transcription factor binding site mapping by DNA affinity purification sequencing (DAP-seq). DNA/gene synthesis should also be requested for construction of affinity-tagged transcription factor clones used in the assay.
- Flow cytometric sorting and genomic analysis of metabolically active microbes labeled via Bio-Orthogonal Non-Canonical Amino acid Tagging (BONCAT)
- Access to high-performance computing at the National Energy Research Scientific Computing Center (NERSC)
- Developing new applications and extending capabilities in KBase
Mechanism and Timing of Review
Letters of intent will only be accepted electronically and should be submitted at https://proposals.jgi.doe.gov/ between February 6 and March 31, 2017. The CSP Call is open to anyone with the understanding that CSP data are made publicly available immediately, without exception. Applicants will be advised by April 14, 2017, whether to prepare a full proposal. Full proposals will be due May 26. Guidance for submitting full proposals will be included in the email notification to invited applicants.
Proposals will be independently peer-reviewed and ranked following given review criteria. Final decisions will be made by DOE JGI senior management with final approval given by DOE program management. All projects will begin as soon as User Agreements are finalized, targeted for October 2017.
For questions about the appropriateness of projects, program specifics or application process, please contact Susannah Tringe.
To respond to the annual CSP call, a Letter of Intent is required before submitting a proposal. Letters of intent for CSP19 will only be accepted electronically and should be submitted at https://proposals.jgi.doe.gov/ between February 6 and March 30, 2018. Applicants will be advised by April 16 whether to prepare a full proposal, and full proposals will be due May 30. Guidance for submitting full proposals will be included in the email notification to invited applicants.
The full FY19 schedule is below:
|Calls for proposals issued||February 6, 2018|
|Letters of intent received||March 30, 2018|
|Invitation of proposals||April 16, 2018|
|Proposals received||May 30, 2018|
|Technical and scientific review||July 2018|
|Approval and rejection notices sent||September 7, 2018|
|Prepare user agreements||September 2018|
|Projects start||As soon as user agreement is finalized|