The objective of this proposal is to understand how biomass-derived carbon is converted into lipids and terpenoids in the oleaginous and carotenogenic yeast Rhodosporidium toruloides. We will carry out a detailed systems biology-level study of wild type and mutant strains using a combination of multi-omic technologies and high resolution microscopy methods. Our goal is to…

“Blue carbon,” or organic carbon (OC) derived from and stored in coastal and marine environments, is recognized as an important OC sink within the global carbon cycle, particularly in mangrove, salt marsh, and seagrass settings. However, as sea level continues to rise, these blue carbon reserves will become increasingly sensitive to wetland erosion/loss, particularly in…

A more complete understanding of how global warming will affect carbon-cycle feedbacks to the climate system is central to model projections of future climate. Simulation experiments with fully coupled, three-dimensional carbon-climate models suggest that carbon-cycle feedbacks could either substantially accelerate or slow climate change over the 21st century. The potential switch of the terrestrial biosphere…

Depositional environments such as floodplains are poorly understood and dynamic components of the global carbon cycle that not are well represented in Earth system models. Further, they have a dominant influence on metal cycling within critical transport conduits to surface and groundwater. Importantly, the fate of carbon and redox active metals such as iron and…

This study will explore the genetics of obligately symbiotic interactions between conifers (Pinaceae) and their host-specific ectomycorrhizal fungi in the mushroom genus Suillus, and their effects on ecological cycling of forest carbon. Using conifer seedlings experimentally inoculated with different Suillus species, we will employ integrated metatranscriptomic / metaproteomic/metabolomic approaches identify key fungal in EMF-Pinaceae roots…

Climate change in the Arctic is progressing rapidly, and much of the focus, thus far, has been on permafrost thaw and the rapid mineralization of soil organic carbon (C) by soil microorganisms. However, climate change is also increasing the abundance of woody deciduous shrubs in the Arctic, altering the vegetative biochemical landscape, which could profoundly…

Coniferous forests are widely distributed in temperate and boreal biomes and the fact that they efficiently store large stocks of carbon makes them globally important. Decomposer and mycorrhizal fungi are key players in the carbon and nitrogen cycles in this environment. Despite the recognition of potential effects of N availability on C cycle-related processes, detailed…

This project aims to produce the first genome assembly of a cultivated sugarcane plant. To date no Saccharum species have been sequenced due to their large complex  polyploid genomes.  Cultivated sugarcane is grown throughout the tropics and sub tropic regions of the world and it produces 80% of the world’s sugar. Sugarcane is a C4 plant…

This project aims to define gene function in rumen microbes to improve our knowledge of carbon (C) flow in the rumen, including the breakdown of lignocellulose, through the metabolism of substrates to volatile fatty acids (VFAs) and fermentation end products, to the final step of methane (CH4) formation. The project builds on the datasets and…

This project would increase the sequence depth across a broader phylogenetic diversity of gymnosperms compared to previous focus on the Pinaceae. The specific transcriptomes and the genome targeted in this project would greatly accelerate our understanding of the functional and structural genomic underpinnings of the diverse secondary metabolic biosynthetic systems of these conifers, and facilitate…