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… went on to work at a groundbreaking company in the early 2000s called Ecopia, which was a very early genome mining … And I set that up. And we finally got that to work 10 years later. But in the meantime there, we had people … a teaspoon of soil from outside, you maybe have to do a 10,000-fold dilution to plate that out. The deeper you go in …

… getting them out to you all. But for now, here is episode 10 of Natural Prodcast, the primer on genome mining. Hey, … found in Yu et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 1253–1262, 70:3 ALISON TAKEMURA: And so did you … Microbiomes. Nature  Biotechnology . 2020 Nov 9. doi: 10.1038/s41587-020-0718-6) DAN UDWARY: Yeah, I think that …

… recruit new talent for biosciences.  Adopt-a-Genome has: ~20 faculty from institutions across the country Efforts in … development and adoption of classroom modules Currently 206 genomes have been selected with 11 published manuscripts … Resour Announc 0:e00891-25. https://doi.org/10.1128/mra.00891-25 . B. Christenson, I. Nouioui, C. Daum, …

… FY2025 Spending Profile The JGI is funded primarily by the DOE …

… seems hard to believe in retrospect. BILL FENICAL: Well, in 2021, absolutely hard to believe. But when you go way back, … convince those people of that fact, however, a long time, 10 years! ALISON TAKEMURA: Wow. It’s all happened so fast. I … of the molecules in ecology. And that took another 10 years at least. But I had several students. And I worked …

… for BERSS. For DNA synthesis requests, the minimum is 100 kb, and for metabolomics, at least 22 samples are … utilize a core facility instead. Proposals may not exceed 10 Tbp sequencing, 500 kbp synthesis, and 200 polar/500 nonpolar metabolomics samples, and should …

… ~50 lineages selected for sequencing 16 genomes released 20 members from 15 institutions 300+ students 800+ …

… to tell a quick story. Looking back, the year was probably 2010, 2011, I was a, uh- actually, I’ve got the t-shirt on … is really happening, if people are really going to sequence 10 or 20 genomes at once then we really need to have …

… We don’t want to get a proposal that’s just asking for 20 microbial genomes. That is not a good fit for our annual … proposals as well. I’ve been at the JGI for 20 years, and 10 of those years, I’ve been a project manager. MENAKA … amount that they can request. So the minimum request is 100 kb. The maximum request is 500 kb. DAN UDWARY: For DNA …

… And you have these different types of particulate like 2.5, 10 PPMs and just the size of the particle and how far they … WINTER: Wow. DAN UDWARY: And I think we’ve got another 200,000 or 300,000 bacteria to go in before we go to the 1.0 … there a way to do that? DAN UDWARY: Well, so there’s good news and bad news on that. So one of the things that we’re …

… So they’re in the picomolar range. ALISON: Picomole… is [10 to the] minus twelve, right? BEN: Yeah, yeah. Well, we … called Mylotag. Wyeth was later acquired by Pfizer in 2009. So, a simple mistake, but I’m happy to be able to … So I wanted to ask you also about – because you were in the news recently – TSRI has acquired the Pfizer strain …