Searching for the basis of ligninolytic selectivity
Many fungal genome projects being carried out at the DOE JGI focus on understanding how enzymes can break down cellulose and lignin, the two most abundant biopolymers on Earth, in order to harness these capabilities for industrial applications such as biofuels production. Scanning electron micrograph of Ceriporiopsissubvermispora mycelium on wood. (Robert Blanchette, University of Minnesota)… [Read More]
Bioinformatics challenges for metagenomic analyses
There are more microbes in, on and around the planet than there are stars in the sky. However, the vast majority of these microorganisms have not yet been studied, in part because many of them do not thrive when moved out of their natural environment. A spoonful of soil contains a complex and diverse microbial… [Read More]
Elucidating bacteria’s roles in ant fungal gardens
Leafcutter ants cultivate fungal gardens that serve as their primary food source. Working toward the goal of harnessing novel enzymes for breaking down plant biomass to produce cellulosic biofuels, Great Lakes Bioenergy Research Center (GLBRC) researchers have been studying the process by which the fungi break down the plant leaves harvested by the ants and… [Read More]
Analyzing enzymes for a PAH degradation pathway
Microbial activity is crucial for breaking down compounds, removing pollutants and chemically transforming organic compounds. Some of these pollutants are polycyclic aromatic hydrocarbons (PAHs) found in contaminated soils. The PAH phenanthrene, for example, can be broken down by the bacterium Arthobacterphenanthrenivorans, which was isolated from a creosote-polluted site in Greece, and used by the microbe… [Read More]
Dietary impacts on hoatzin crop microbial communities
Many DOE JGI metagenomic projects focus on microbial communities in the guts of the cow, termite and even the desert locust, all known to break down plant biomass for energy. In studying these and other gut microbial communities, researchers hope to identify and isolate genes involved in plant biomass degradation, and apply them to biofuel… [Read More]
A new approach for improve genome assembly
Assembling a genome from fragments of DNA sequence is often compared to assembling a puzzle. One of the problems researchers face with the increasing use of next-generation sequencing technologies is that the pieces of DNA sequence generated by the 454 or Illumina platforms are much smaller and far more numerous than those produced by the… [Read More]
The role of hydrophobins in plant-fungal mutualism
A toolkit for T. reesei
The availability of an organism’s genome sequence is useful for improving downstream applications such as large-scale biofuel production, but it is only the first step on this path. In the case of the fungus Trichoderma reesei, whose genome sequence was published by the DOE JGI in 2008, the cellulases in T. reesei have multiple industrial… [Read More]
Cyanobacteria shed light on carboxysome complexity
Found in temperate and tropical oceans, Prochlorococcus cyanobacteria are considered the world’s most abundant photosynthetic organisms, able to convert sunlight to energy at depths of 200 meters. Despite their size, they are estimated to contribute up to half of the marine biological carbon sequestration. Prochlorococcusis a unicellular cyanobacterium that dominates the temperate and tropical oceans. … [Read More]