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High Throughput Phosphoglycolate Phosphatase Activity Assay Using Crude Leaf Extract and Recombinant Enzyme to Determine Kinetic Parameters Km and Vmax Using a Microplate Reader
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Help from Citrus’ Bitter, Hardier Cousin
… The chromosome-scale genome sequence of trifoliate orange yields clues to disease-resistance that … from greening disease. You’ve probably never heard of trifoliate orange. Otherwise known as  Poncirus … may be a plant of bitter fruit, yet sweet are its genetic secrets. This work builds upon JGI-supported research led by …
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A Powerful Technique to Study Microbes, Now Easier
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Game On: High School Students Drive Everglades Metagenomics Study
… In late June, Kathleen Lail, a member of JGI’s Sample Management Group, attended a conference in … in the Florida Everglades to help train the next generation of scientists. She shares her thoughts about the experience … course had a defined set of objectives, and started with an image of a spoonful of dirt with the question: Do you want …
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Model Fern Reveals Insight into DNA Thievery in Ferns
… — making it difficult to grasp a deeper understanding of fundamental aspects of fern biology and, more broadly, … to protect themselves from environmental stresses, ferns’ secondary metabolites and associated genes are great resources for … The work conducted by the US Department of Energy Joint Genome Institute is supported by the Office …
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Corymbia Genome Expands Terpene Synthesis Knowledge
… Genome annotations of two C. citriodora subspecies broaden understanding of the … suggest that these plants synthesize a high level of secondary metabolites which play a part in biotic and abiotic stress … Sequencing and assembly data carried out by EMBRAPA as part of the  Corymbia  genome project was supported by FAPDF …
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An Enzyme System to Help Catalyze Bioproduction of Plastic
… By characterizing the structure and function of a methylthio-alkane reductase system, researchers could … more.  Microbes excel at recycling the abundant miscellanea of their surroundings. Case in point: for us, the smell of the ocean simply signals we’re near the beach. For …
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Doubling Down on Known Protein Families
… online Oct. 11, 2023 in  Nature highlights the vast array of functional diversity of microbes through a novel approach to better understand … “So, that diversity is definitely holding a lot of new secrets in terms of functional diversity as well.”  … The U.S. Department of Energy Joint Genome Institute, a DOE Office of Science …
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A Pinch of Salt Activates Silent Gene Clusters in Streptomyces
… chemical factories. They hold genetic blueprints for dozens of complex compounds, but stay largely dormant under … Summary Most Streptomyces species can produce roughly 30 secondary metabolites — chemical compounds not required for basic … This work conducted by the U.S. Department of Energy Joint Genome Institute …
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Soil virus offers insight into maintaining microorganisms
… The work was enabled in part by a community data portal of the U.S. Department of Energy (DOE) Joint Genome … Berkeley National Laboratory (Berkeley Lab). Using 5,000 images of crystalized samples cloned and synthesized by the … have begun the complex process of uncovering the hidden secrets of soil viruses. The Impact The specific protein …
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FICUS
… for researchers to request the capabilities and expertise of multiple DOE National User Facilities in one research … Successful applications will focus on high-risk/high-payoff proposals within the focused topic areas that can be … CSP projects. In addition, they must utilize a broad range of the capabilities across at least two of the facilities, …
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A Step Toward Controlling Soybean Rust
… causes Asian Soybean Rust (ASR) and is the major pathogen of soybean. Left uncontrolled, soybean rust could reduce crop yields by as much as 90 percent. As part of the DOE Joint Genome Institute’s  2016 Community Science Program portfolio , Sebastien Duplessis of the French National Institute for Agricultural Research …
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