The Sequencing Technologies Group applies cutting edge molecular capabilities and state-of-the-art next-generation sequencing to enable biological discovery by the JGI and external DOE users. Adopting the latest sequencing technologies has allowed the JGI to increase sequencing output while significantly lowering costs.
Staff process samples, perform quality checks, and prep libraries for sequencing. They apply Lean Six Sigma practices to process optimization and development approaches, testing various platforms to see what works best for applications. The team works hand in hand with the Project Management Office to help ensure the JGI’s genomic capabilities are ready and applicable to users.
Sequencing Process
Sample receipt
Quality Check
Library Construction
Sequencing
Portfolio
Library Capabilities
- Genome: DNA
- For genome assembly, structural variation, genetic diversity (MAGs), and resequencing
- Transcriptome: RNA
- For gene annotation, metagenome discovery, and gene expression
- Epigenome: DNA-protein interactions
- For methylation, genome structure, gene regulation, and transcription factor binding
Sequencing Platforms
- Illumina NovaSeq X Plus: the JGI’s primary sequence generator
- Illumina MiSeq: for Library QC and R&D
- PacBio Revio: for assembly and methylation detection
- Oxford Nanopore PromethION and MinION: for R&D, Benchmarking and Validation
