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… are complex organisms that harbor a fantastic diversity of cell types and associated organisms, including fungal and … are influenced by microbial partners. We use a variety of genomics-powered tools to study this complexity, including single-cell and spatial transcriptomics barcode-based …

… on DNA sequencing and synthetic biology. But also the metagenomics and gut microbiome, as I think that it’s like a … is always associated with toxicity, basically, killing cells. ALISON: Right, because they’re so reactive. BEN: … DAN: Yeah, I don’t think your lab is gonna tackle that single handedly, right? So, yeah, getting the community …

… predict or just understand better the language of microbial cell-cell communication. And he told us about his work in … bound to it and maybe there will be some a affinity tag or a fluorescent tag, and so you can use that as a … and they were able to synthesize the genes for every single one of those and put them in individual plasmids and …

… a great opportunity to learn more about JGI, hear some excellent talk from our users and from other invited guests, … in an obvious way. NIGEL MOUNCEY: We did a little bit of metagenome screening work and did find related clusters. But … and the stabilized isotope probing work that we’re doing, single cell work we’re doing. And using these together along …

… streams. This will all become clearer as we get into the genomics. So– ALISON TAKEMURA: I’m just enjoying the ride … found in Yu et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 1253–1262, 70:3 ALISON TAKEMURA: And so did you … if an order is uncultured (blue; represented only by metagenome-assembled genomes or MAGs) or cultured (gray; …

… come, though, too when you start talking about going after single gene clusters. BRIAN BACHMANN: That’s right. JACKIE … mammalian systems. But it allows us to assay on a single cell level against cell lines and against primary cells … by chemical synthesis and make analogs in combination of mutagenesis and direct evolution. Yeah. DAN UDWARY: So it’s …

… types) (Nature Communications 2017, Science 2018, Molecular Cell 2019) and massive reconstruction of genomes from … Unraveling the functional dark matter through global metagenomics. Nature . 622(7983):594-602. Camargo AP. et al. … Bowers RM, et al. (2017) Minimum information about a single amplified genome (MISAG) and a metagenome-assembled …

… interpretation of sequence, metabolomics, and functional genomics data types as well as in advanced assembly and … Proposal DOIs can be found at the top of the proposal info page on the JGI Genome Portal. You can navigate to the … for your proposal using your proposal's WIP ID (ex. 503582). Click on the correct proposal name on your search …

… quantities of DNA derived from individual environmental cells. The proof of principle study built off earlier … and finished genome derived from an uncultured bacterial single cell – a symbiont of the green sharpshooter. In 2012, … of the tree of life. Used in tandem over the years, metagenomics and single cell genomics have afforded JGI users …

… of ground – her book, bioprospecting vs biopiracy, early stage drug discovery in general, and a possible data-driven … has a book called The Plant Hunter and I’ve read all 400 pages of her life and I feel like I have a new friend that … and other natural product libraries, so not relying on single crystal X-ray diffraction, but looking actually at …

… available from all the facilities participating in the FY25 call, introduces the resources available from the … useful tips for submitting a successful proposal. … The FY23 FICUS Open Call for Proposals describes the resources … Accessing Resources at Multiple DOE User Facilities with a Single Proposal describes the resources available from the …

… community as a user facility pioneering functional genomics to solve the most relevant bioenergy and … the Letter of Intent; rather proposals are submitted in a single step. … The researcher then submits a proposal for …