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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… Studying the  genomes of microorganisms is incredibly useful for understanding the microbiome of the earth, but … The U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User Facility located at … like plasmids, the picture is far from complete.  In 2021 , scientists at St. Jude Children’s Research Hospital …
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Mapping Heat Resistance in Yeasts
… species to grow better than its closest relative S. paradoxus at high temperatures (39°C/102°F). The Impact One of the challenges in understanding … Genomics Project led by the DOE Joint Genome Institute (JGI), a DOE Office of Science User Facility of Lawrence …
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A Natural Mechanism Can Turbocharge Viral Evolution
… The Science The first step in the deadly dance between a virus and microbial cell is an embrace. For a virus with a “head-tail” morphology, this means using proteins … the US Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science user facility located at …
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New and Improved: Eucalyptus Reference is Now Accessible
… Researchers report a new reference genome for eucalyptus in the July edition of the journal G3 Genes | Genomes | Genetics , with significant advances compared to the 2014 Eucalyptus grandis reference genome that has been widely … The new improved genome assembly generated through the JGI’s Community Science Program will help support targeted …
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Effects of Polar Light Cycle on Microbial Food Web
… scant, Ace Lake’s bacteria instead go through a boom and bust cycle aligned with light availability. A long-running … cycles. Water samples from Ace Lake were collected over a 10-year period and sent to JGI for metagenomic sequencing. A study  recently published …
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A Complex Confection Decoded: Sugarcane Reference Genome
… the location of genes that provide resistance to the brown rust disease that can devastate a crop. The Impact In providing a reference sugarcane  genome , the JGI provides foundational genetic information that crop … partly enabled by the JGI’s Community Science Program; in 2018, a monoploid reference sequence of sugarcane …
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Mining Metagenomes for Cas Proteins
… Cas14 proteins discovered from JGI’s IMG/M database and biochemically characterized at UC … The Science Researchers report the discovery of miniature Clustered Regularly Interspaced Short Palindromic Repeats … The work conducted by the US Department of Energy Joint Genome Institute, a DOE Office …
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Streamlining Regulon Identification in Bacteria
… sigma factors that initiate transcription in bacteria. Using this technology, which combines an in vitro … sequencing, they were able to identify the target genes of 11 sigma factors in Streptomyces coelicolor . The Impact As … the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User Facility located at …
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From Stress to Growth: The adaptive mechanisms of algae in changing Arctic waters
… yield and climate resilience of algae — for potential use as biomass or in biomass conversion to biofuel. Summary … dead cells recorded. Samples were taken again at two, six, 12 and 24 hours. By the 24-hour mark, these algae showed … the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User Facility located at …
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Ancient Algae Reveal Secrets of Plant Evolution
… to terrestrial life paves the way for innovations in sustainable agriculture and clean energy. These evolutionary … in marginal lands and with minimal resources. Enabling the use of non-arable land for biomass production — that can be … the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User Facility located at …
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Busting the Unbreakable Lignin
… The Impact Traditional methods for breaking down biomass use aerobic methods (those using oxygen) to remove lignin … all life forms and with little exception, organisms have figured out how to create enzymes that break these … Research Center. O’Malley is also affiliated with JBEI. The JGI data portals and pipelines helped identify potential …
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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
… A group led by JGI scientists have made strides in improving and applying a … to quickly modify diverse bacterial genomes. In 2019, the JGI’s Yasuo Yoshikuni and his team  announced in  … is central to our ability to offer host-engineering for our users,” says JGI Director Nigel Mouncey. Using CRAGE, JGI …
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