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Boosting Small Molecule Production in Super “Soup”
… Do metabolic pathways from engineered yeasts still work in cell-free … the sugar glucose to the products including ethanol and 2,3-butanediol (BDO). To do so, the yeasts received … one of the study co-authors, have maintained a regular shipment schedule, with plates arriving every couple of months. …
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Engagement Webinar: Accessing NEON’s Environmental Sample Archives, Applying JGI & EMSL Omics Tools
… The  National Ecological Observatory Network (NEON) is a network of field research sites … which archives and makes available for research over 100,000 samples and specimens of over 60 different types of … Genome Institute, a DOE Office of Science User Facility  located at Lawrence Berkeley National Laboratory, is committed …
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Identifying the Microbial Cleanup Crew at a Superfund Site
… reveals the microbial community involved in a bioremediation effort. The Science In 1999, the Environmental … can help bioremediate toxic substances has led to the development of cost-effective environmental cleanup strategies. … samples from wells at the site in 2007, 2009, and 2010. DNA was extracted from the samples that were sequenced …
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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… microbes , scientists usually employ two different methods; shotgun metagenomic sequencing and single-cell sequencing. … is through a method called Multiple Displacement Amplification, and most genomes that were acquired through it were usually only around 30% complete.  Described in  ISME Communications , a method …
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Forming Scientific Connections and Building Community
… DOE Office of Science User Facility, hosted three undergraduates and seven graduate students from UC Merced through their long standing … Mentors: Deze Kong & Yasuo Yoshikuni Project: “ Development of a multiplex genomic integration system for the …
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Marine Microbe Contains Multitudes
… with different lifestyles. The Science The bacterium SAR324 is unusually cosmopolitan. In the ocean’s North Pacific Subtropical Gyre, microbes tend to stay localized at different depths. But SAR324 can be found throughout the water column, from the warm, well-lit surface, to the …
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An Enzyme System to Help Catalyze Bioproduction of Plastic
… bacteria to produce ethylene and more.  Microbes excel at recycling the abundant miscellanea of their surroundings. … ocean simply signals we’re near the beach. For bacteria, that salty, slightly stinky air is a rich source of the sulfur … need to grow and divide. Similar to the bacterial enzymes that fix nitrogen — nitrogenases — other enzymatic machinery …
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Secrets of the Needles: What Microbes do on Conifer Leaves
… Novel study looks at microbiomes in aerial surfaces of plants and their roles … on root-associated microbiomes. Scientists conducted a shotgun metagenomic survey on three types of conifers–limber … of conifer needle microbiomes across six sites spanning a 1000km section of the Rocky Mountains. This represents the …
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What Happens Underground Influences Global Nutrient Cycles
… DOE user facilities EMSL and JGI announce FY 2020 collaborative FICUS projects. Through the Facilities Integrating Collaborations for User Science (FICUS) program, two … (JGI)—have selected 11 proposals for support from 53 received through a joint research call. Submitted …
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Len Pennacchio
… In 2003, Dr. Pennacchio joined the DOE Joint Genome Institute and has focused on applying state-of-the-art molecular methods to address wide-ranging … biology. He holds joint appointments as a Deputy Director at the DOE Joint Genome Institute, Senior Scientist at …
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Engagement Webinar: Harnessing JGI’s Metabolomics Capabilities
… The JGI Engagement Webinar held November 5, 2020 (watch the  video below, captions are available) spotlighted … . ACS Chem Biol. 2019 Apr 19;14(4):704-714. doi: 10.1021/acschembio.8b01107. Epub 2019 Apr 4. Q:  Any advice on … Genome Institute, a DOE Office of Science User Facility located at Lawrence Berkeley National Laboratory, is committed …
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Uncovering Novel Genomes from Earth’s Microbiomes
… Large-scale comparative analysis leads to identification of biosynthetic gene … Using this massive dataset, Nayfach clustered the MAGs into 18,000 candidate species groups, 70% of which were novel … BGCs in IMG/ABC (Atlas of Biosynthetic Gene Clusters) by 31%. ( Listen to this JGI Natural Prodcast episode on genome …
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