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Streamlining Regulon Identification in Bacteria
… sigma factors that initiate transcription in bacteria. Using this technology, which combines an in vitro … coelicolor . The Impact As an integrative genome science user facility, connecting genes to functions is part of the … of RIViT-seq to other transcription factors and even other organisms can fuel a deeper understanding of these …
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Protocols
… Find protocols provided by JGI and the JGI user community below for DNA preparation tools, high …
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THE Bioenergy Tree
… The US Department of Energy’s favorite tree is poplar. They’re … who have uncovered remarkable genetic secrets that bring us closer to making poplar an economical and sustainable source of energy and materials. …
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Graduate Students Get Thesis Research Opportunity at the JGI
… at one of the DOE national laboratories or national user facilities. As a result of the latest call , a total of … UC Merced School of Natural Sciences. “I’m deeply concerned about climate change and this is a remarkable opportunity to … how engineered environments contribute to greenhouse gases,” Kaze said. “It’s an exciting time in the field …
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Designer DNA: JGI Helps Users Blaze New Biosynthetic Pathways
… (DOE) Joint Genome Institute (JGI), a DOE Office of Science User Facility located at Lawrence Berkeley National … mining, empowering research collaborators to take on ambitious synthetic biology experiments. A recent special issue of … helped LanzaTech researchers tame gene expression in the organism Clostridium autoethanogenum. The microbe can …
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Mike Sneddon
… a laboratory information management (LIMS) system. He previously led the NGS Software team and metagenomic data platform … discovery platform. WO2021163637A1 , PCT application PCT/US2021/018050 (published 2021). Arkin, AP, et al. KBase: The … Nature Biotechnology 36, 566–569 (2018). Sneddon, MW, Pontius, W, and Emonet, T. Stochastic coordination of multiple …
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A Better Way to Find RNA Virus Needles in the Proverbial Database Haystacks
… a computational pipeline to sift and characterize RNA virus sequences.  A zoo once offered a coloring book featuring … polar bears in winter scenes that came with crayons in various shades of white. To researchers searching for sequences … absence can impact the planet’s nutrient cycles. While most organism’s genetic information is encoded in DNA, with RNA …
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Discovery and ecogenomic context of a global Caldiserica-related phylum active in thawing permafrost, Candidatus Cryosericota phylum nov., Ca. Cryosericia class nov., Ca. Cryosericales ord. nov., Ca. Cryosericaceae fam. nov., comprising the four species
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Better Genome Editing for Bioenergy
… gene-editing tool that can help scientists engineer organisms for bioenergy applications. Cas9 needs guide RNA … of the genes in a lipid-prolific yeast. The Impact The team used this set of guide RNAs on  Yarrowia lipolytica to … furthers the U.S. Department of Energy’s goal of producing sustainable fuels from alternative sources. Moreover, the …
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Massie S. Ballon
… as help cultivate the next generation of genomic research users, partners, and collaborators.  Ms. Ballon is a … to see the coverage of JGI-enabled science highlighting user research, newly accepted proposals, and outreach … The Manager of Communications & Outreach is responsible for sustaining the integrity of the JGI brand through an …
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JGI-Enabled Research Demonstrates the Existence of New Methane-Makers
… New studies show methanogenesis is not exclusively for Euryarchaeota. Atmospheric levels of methane, a … through a process called methanogenesis. Methanogenic microorganisms therefore play a critical role in the global … Alternate Metabolic Pathways And there is something special about these new lineages recently tied to methanogenesis. …
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Strain Engineering
… The focus of this group, in recent years, has been to bring … technologies to bacteria, yeasts and algae previously with little to no genetic tools enabling heterologous expression and functional exploration.  The developed …
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