Find protocols provided by JGI and the JGI user community below for DNA preparation tools, high molecular-weight DNA extraction and RNA preparation. JGI sample QC protocols can be found here.DNA Extraction Protocols: JGI Bacterial DNA isolation CTAB-2012 Isolation of Genomic DNA from Phytophthora Isolation of Melampsora Nuclear DNA DNA Isolation of Plant Nuclear DNA Populus nuclear DNA purification with Qiagen DNA extraction from Activated Sludge Cloning of High Molecular Weight eDNA (soil) RNaseA DNA Cleanup eDNA purification (removal of humics) High Molecular Weight DNA Extraction from Soil 1000 Fungal Genomes protocols High Molecular Weight DNA extraction protocols from PacBio Tips for High Molecular Weight DNA extraction: DNA Extraction 101 Sample Collection & Storage Guidance Best Practices Click for Pac Bio's best practices for High Molecular Weight DNA extraction and quality control. RNA Extraction Protocols: DNase Treatment of Total RNA LiCl RNA cleanup RNA Isolation from LCM samples Chlamydomonas large-scale RNA extraction JGI RNASeq data – Lane to lane variation related to differential expression The JGI has conducted studies to verify that technical replicates are not needed for RNASeq experiments. Click to see the results of those studies detailed in this Decemeber 2013 presentation. iTag protocols: Amplification QC iTag library construction Primer Sequences
