Find protocols provided by JGI and the JGI user community below for DNA preparation tools, high molecular-weight DNA extraction and RNA preparation. JGI sample QC protocols can be found here.
DNA Preparation Tools:
JGI Bacterial DNA isolation CTAB-2012
Isolation of Genomic DNA from Phytophthora
Isolation of Melampsora Nuclear DNA
DNA Isolation of Plant Nuclear DNA
Populus nuclear DNA purification with Qiagen
DNA extraction from Activated Sludge
Cloning of High Molecular Weight eDNA (soil)
RNaseA DNA Cleanup
eDNA purification (removal of humics)
High Molecular Weight DNA Extraction from Soil
1000 Fungal Genomes protocols
High Molecular Weight DNA extraction protocols from PacBio
Tips for High Molecular Weight DNA extraction:
DNA Extraction 101
Tips, kits and protocols
Sample Collection & Storage Guidance
RNA preparation protocols:
DNase Treatment of Total RNA
LiCl RNA cleanup
RNA Isolation from LCM samples
Chlamydomonas large-scale RNA extraction
iTag protocols:
Amplification QC
iTag library construction
Primer Sequences
