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Secondary Metabolites Program focuses on the exploration of secondary metabolites across bacteria, fungi, algae and … cell free-based expression platforms, tools and data for deciphering regulatory networks, and refining experimental … by their native hosts. We are developing cell-free based systems to express BGCs that alleviate the constraints of …

… … DAN UDWARY: You’re listening to the Department of Energy Joint Genome Institute’s Natural Prodcast– a … one metabolite. They produce a large array of different molecular species related– chemically related– but … of natural product biosynthesis in bacteria ” The ActDES publication came out after we talked. It looks like a great …

DAN UDWARY: You’re listening to the Department of Energy Joint Genome Institute’s Natural Prodcast, a … but also those molecules that mediate a well functioning ecosystem and environment and earth. So yeah, it’s like these … and how we do it here and maybe give an example of a recent publication. ALISON TAKEMURA: Awesome. Yeah, let’s dive in, …

Below is a brief description of the process needed to prepare and ship samples for sequencing by the JGI.  Additionally it includes instructions on … and storage (from PacBio) Best practices for High Molecular Weight DNA extraction and quality control (from …

… actual molecules present in an environment that influence system behavior.  Through this identification, metabolomics … functional genome annotation, improves understanding of the role small molecules play in diverse biological …   Overview of metabolomics products At JGI, in-depth molecular profiles are generated for metabolites extracted …

… here today, and we wanted to talk to her about a whole list of things. So we have a Scripps background shared together, … we want to talk about Antarctica, and we want to talk about molecular networking, because I really enjoyed the review … is a whole bunch of information that maybe never makes its publication. And these are little tips, you know, how to …

… for researchers to request the capabilities and expertise of multiple DOE National User Facilities in one research … provides customized analysis, the JGI also participates in publication of results. … Proposals for the annual FICUS … at Oak Ridge National Laboratory  Center for Structural Molecular Biology . … Accessing NEON’s Environmental Sample …

Find protocols provided by JGI and the JGI user community below for DNA preparation tools, high molecular-weight DNA extraction and RNA preparation.  JGI … be found here . DNA Extraction Protocols: … Tips for High Molecular Weight DNA extraction: … RNA Extraction Protocols: …

… research is focused on the discovery and characterization of novel bacterial, archaeal and eukaryotic microbes and … and biogeochemical cycles. Research Team    … A complete publication list available at Google Scholar. Schulz, C … viruses with an expanded complement of translation system components. Science, 356 (6333), 82-85 Schulz et al. (2016) …

… … DAN: You’re listening to the US Department of Energy Joint Genome Institute’s “Natural Prodcast,” a … fungi were transformed. So I wasn’t even thinking of molecular genetics of finding genes when I first started … challenges were associated with getting good transformation systems going. That has a lot to do with, well, the fact …

… … DAN: You’re listening to the US Department of Energy Joint Genome Institute’s “Natural Prodcast,” a … product hits. Sort of in parallel with that, the field of molecular biology got more sophisticated around the 80s and … So you slowed everything down by introducing these foreign components. Because we still don’t quite understand the …

Tracing gene regulatory networks in the model fungus  Neurospora … . The Science A team led by researchers at the University of California, Berkeley used a multi-omics approach to … efficiently break down the wide array of simple and complex components present in plant biomass. This is of interest for …