Published in:
Int J Syst Evol Microbiol 67(10) , 3752-3758 (Oct 2017)
Author(s):
DOI:
10.1099/ijsem.0.002184
Abstract:
Strain EPL6(T), a Gram-negative, motile, short rod was isolated from a propanil and 3,4-dichloroaniline enrichment culture produced from rice paddy soil. Based on the analyses of the 16S rRNA gene sequence, strain EPL6(T) was observed to be a member of the family Comamonadaceae, sharing the highest pairwise identity with type strains of the species Alicycliphilus denitrificans K601(T) (96.8 %) and Melaminivora alkalimesophila CY1(T) (96.8 %). Strain EPL6(T) was able to grow in a temperature range of 15-37 degrees C, pH 6-9 and in the presence of up to 4 % (w/v) NaCl and tested positive for catalase and oxidase reactions. The major respiratory quinone was Q8. The genomic DNA had a G+C content of 69.4+/-0.9 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and the major fatty acid methyl esters comprised C16 : 0, C18 : 1omega7c and summed feature 3 (C16 : 1omega7c/iso-C15 : 0 2-OH). Comparison of the genome sequence of strain EPL6(T) and of its closest neighbours, Melaminivora alkalimesophila CY1(T) and Alicycliphilus denitrificans K601(T), yielded values of ANI View Publication