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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … lots of stuff. On average, we had something– I think we had two biosynthetic clusters per MAG, which is pretty good …

Moving forward, I’m hoping to get a new one out every two weeks. This time, we’ve got a great interview with Ben … the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … is what. This is not new. It actually was first proposed by two authors called Firn and Jones. I’m sure that people … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … sending signals. But really unpacking that complicated network of chemistry and communication is what I’m really …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … gene classes I could start on. So… but you started with two genomes, right? DAN UDWARY: Right. NADINE ZIEMERT: And … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… welcome back to a new session of Natural Prodcast. I’ve got two things I wanted to tell you about in this introduction. … And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, …

I’m interested in the world of natural products is also twofold but for different reasons to learn about how nature … enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … Proposals may be submitted at any time. Support is limited to work included in the BER-funded proposal, and PIs …  should fill out this form . Please allow a minimum of  two weeks for your request to be processed. Investigators …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …

DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … – how bacteria or other organisms communicate and how and why secondary metabolites are being produced in the … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …