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Sequencing Submission Requirements for DNA Synthesis
When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …
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Natural Prodcast Episode 12: Nadine Ziemert
Prodcast. And it’s our conversation with Nadine Ziemert from the University of Tübingen in Germany. Like me, she’s a … there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …
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Sequencing Technologies
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Natural Prodcast Episode 13: Francisco Barona Goméz
… prefers to be known among friends in natural products. He’s from Langebio in Irapuato, Mexico, and an incredibly bright … least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …
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Products
… the product and deliverables as well as target cycle times from sample receipt to completion of standard analysis.  Raw … sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs …
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Publication Policy
… (see Auspice Statement below) for the generation of sequence or metabolomic data, DNA synthesized, and any other … is done in a timely fashion. The publications resulting from such efforts should specify the collaborative nature of … annotation, comparative analysis, and interpretation of sequence, metabolomics, and functional genomics data types …
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Natural Prodcast Episode 22: Alison Narayan
… we have our conversation with Professor Alison Narayan, from the University of Michigan. I hope she’ll accept this … enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh …
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Natural Prodcast Episode 10: A Primer on Genome Mining
… genome mining on more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …
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How to Submit Sequencing Samples
QC Protocol DNA Extraction 101 : tips, kits, and protocols (from PacBio) Guidance for sample collection and storage (from PacBio) Best practices for High Molecular Weight DNA … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …
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Mission
…     Our homepage video includes external footage from:  University of Delaware Laura.Selbmann©PNRA. All the … in doing so enabled the JGI to be the first to publish the sequence analysis of the target chromosomes 5, 16, and 19, … cycles, as well as atmospheric particle formation and evolution.  Data from such studies is expected to advance a …
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Natural Prodcast Episode 7: Ben Shen
This time, we’ve got a great interview with Ben Shen, from the Scripps Research Institute in Jupiter Florida. This … the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very …
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Natural Prodcast Episode 20: Brian Bachmann
… our interview this week is with Professor Brian Bachmann from Vanderbilt University’s Chemistry Department, where he … And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, …
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