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Sequencing Submission Requirements for DNA Synthesis
When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …
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Sequencing Technologies
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Microbial Program
… laboratory isolation and genome sequencing of underexplored bacterial and archaeal lineages that are important for … resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … and function distribution. These are derived from isolates and single cells. This effort uncovers novel genes, …
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New Lineages of Life Group
… is focused on the discovery and characterization of novel bacterial, archaeal and eukaryotic microbes and viruses in environmental sequence data. We use multi-omics (metagenomics, … 1-9 Schulz et al. (2017) Towards a balanced view of the bacterial tree of life. Microbiome. 5 (1), 1-6 Schulz et al. …
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Partnerships: Adopt-A-Genome
… in the analysis and publication of the claimed genome sequences. This opportunity, provided by the Department of … sequence to biological inference. … Genome sequences of key bacterial symbionts of entomopathogenic nematodes: … 2023 12 (10), e00548-23 Whole genome sequence of the denitrifying thermophile Geobacillus thermodenitrificans …
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Plant-Microbial Interactions Group
… cell types and associated organisms, including fungal and bacterial symbionts, detrimental microbial pathogens, and … viruses. Resident viruses can be found in a myriad of bacterial strains, whereby they can modulate host … function of these phage genes is the modulation of their bacterial host to colonize plant roots, as with Arabidopsis …
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Products
… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …
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Natural Prodcast Episode 21: Elizabeth Parkinson
… about several different pieces of her work, including bacterial quorum sensing and its effects on regulation of … The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain …
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Natural Prodcast Episode 7: Ben Shen
… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … this collection in total contains approximately 100,000 bacterial strains, and a hundred thousand fungal strains. …
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Natural Prodcast Episode 10: A Primer on Genome Mining
… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … doi: 10.1038/s41587-020-0718-6) The GEM catalog expands the bacterial and archaeal orders as seen on the phylogenetic …
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Natural Prodcast Episode 13: Francisco Barona Goméz
… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … BARONA-GOMEZ: Yeah, so any metabolite, produced by bacterial or fungal metabolism by microbes, will have a … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …
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How to Submit Sequencing Samples
… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …
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