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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  …

… … DAN: You’re listening to the US Department of Energy Joint … the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very …

… … DAN: Hey Marcy!  ALISON: Hey Marcy?  MARCY: Hello. How’s it … does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

The Sequencing Technologies Group applies cutting edge molecular capabilities and state-of-the-art next-generation sequencing …

… … DAN: You’re listening to the US Department of Energy Joint … out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk …

… … DAN: You’re listening to the US. .Department of Energy Joint … at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways …

DAN UDWARY: You’re listening to the Department of Energy … more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… … DAN: You’re listening to the US Department of Energy Joint … stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … susceptible to a fungal infection of rye grain – a specific fungus, Claviceps purpurea, can infect grain. And when it …

… … DAN: You’re listening to the US Department of Energy Joint … DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

The JGI has diverse capabilities in: DNA and RNA sequencing, Sample … sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs …

… … DAN UDWARY: You’re listening to the Department of Energy … least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

Below is a brief description of the process needed to prepare and ship samples for sequencing by the JGI. … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …