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    Data yielded from RIViT-seq increased the number of sigma factor-gene pairs confirmed in Streptomyces coelicolor from 209 to 399. Here, grey arrows denote previously known regulation and red arrows are regulation identified by RIViT-seq; orange nodes mark sigma factors while gray nodes mark other genes. (Otani, H., Mouncey, N.J. Nat Commun 13, 3502 (2022). https://doi.org/10.1038/s41467-022-31191-w)
    Streamlining Regulon Identification in Bacteria
    Regulons are a group of genes that can be turned on or off by the same regulatory protein. RIViT-seq technology could speed up associating transcription factors with their target genes.

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    (PXFuel)
    Designer DNA: JGI Helps Users Blaze New Biosynthetic Pathways
    In a special issue of the journal Synthetic Biology, JGI scientific users share how they’ve worked with the JGI DNA Synthesis Science Program and what they’ve discovered through their collaborations.

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    A genetic element that generates targeted mutations, called diversity-generating retroelements (DGRs), are found in viruses, as well as bacteria and archaea. Most DGRs found in viruses appear to be in their tail fibers. These tail fibers – signified in the cartoon by the blue virus’ downward pointing ‘arms’— allow the virus to attach to one cell type (red), but not the other (purple). DGRs mutate these ‘arms,’ giving the virus opportunities to switch to different prey, like the purple cell. (Courtesy of Blair Paul)
    A Natural Mechanism Can Turbocharge Viral Evolution
    A team has discovered that diversity generating retroelements (DGRs) are not only widespread, but also surprisingly active. In viruses, DGRs appear to generate diversity quickly, allowing these viruses to target new microbial prey.

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    Photograph of a stream of diatoms beneath Arctic sea ice.
    Polar Phytoplankton Need Zinc to Cope with the Cold
    As part of a long-term collaboration with the JGI Algal Program, researchers studying function and activity of phytoplankton genes in polar waters have found that these algae rely on dissolved zinc to photosynthesize.

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    This data image shows the monthly average sea surface temperature for May 2015. Between 2013 and 2016, a large mass of unusually warm ocean water--nicknamed the blob--dominated the North Pacific, indicated here by red, pink, and yellow colors signifying temperatures as much as three degrees Celsius (five degrees Fahrenheit) higher than average. Data are from the NASA Multi-scale Ultra-high Resolution Sea Surface Temperature (MUR SST) Analysis product. (Courtesy NASA Physical Oceanography Distributed Active Archive Center)
    When “The Blob” Made It Hotter Under the Water
    Researchers tracked the impact of a large-scale heatwave event in the ocean known as “The Blob” as part of an approved proposal through the Community Science Program.

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    A plantation of poplar trees. (David Gilbert)
    Genome Insider podcast: THE Bioenergy Tree
    The US Department of Energy’s favorite tree is poplar. In this episode, hear from ORNL scientists who have uncovered remarkable genetic secrets that bring us closer to making poplar an economical and sustainable source of energy and materials.

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    HPCwire Editor's Choice Award (logo crop) for Best Use of HPC in the Life Sciences
    JGI Part of Berkeley Lab Team Awarded Best Use of HPC in Life Sciences
    The HPCwire Editors Choice Award for Best Use of HPC in Life Sciences went to the Berkeley Lab team comprised of JGI and ExaBiome Project team, supported by the DOE Exascale Computing Project for MetaHipMer, an end-to-end genome assembler that supports “an unprecedented assembly of environmental microbiomes.”

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    With a common set of "baseline metadata," JGI users can more easily access public data sets. (Steve Wilson)
    A User-Centered Approach to Accessing JGI Data
    Reflecting a structural shift in data access, the JGI Data Portal offers a way for users to more easily access public data sets through a common set of metadata.

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    Phytozome portal collage
    A More Intuitive Phytozome Interface
    Phytozome v13 now hosts upwards of 250 plant genomes and provides users with the genome browsers, gene pages, search, BLAST and BioMart data warehouse interfaces they have come to rely on, with a more intuitive interface.

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    screencap from Amundson and Wilkins subsurface microbiome video
    Digging into Microbial Ecosystems Deep Underground
    JGI users and microbiome researchers at Colorado State University have many questions about the microbial communities deep underground, including the role viral infection may play in other natural ecosystems.

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    Yeast strains engineered for the biochemical conversion of glucose to value-added products are limited in chemical output due to growth and viability constraints. Cell extracts provide an alternative format for chemical synthesis in the absence of cell growth by isolating the soluble components of lysed cells. By separating the production of enzymes (during growth) and the biochemical production process (in cell-free reactions), this framework enables biosynthesis of diverse chemical products at volumetric productivities greater than the source strains. (Blake Rasor)
    Boosting Small Molecule Production in Super “Soup”
    Researchers supported through the Emerging Technologies Opportunity Program describe a two-pronged approach that starts with engineered yeast cells but then moves out of the cell structure into a cell-free system.

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    These bright green spots are fluorescently labelled bacteria from soil collected from the surface of plant roots. For reference, the scale bar at bottom right is 10 micrometers long. (Rhona Stuart)
    A Powerful Technique to Study Microbes, Now Easier
    In JGI's Genome Insider podcast: LLNL biologist Jennifer Pett-Ridge collaborated with JGI scientists through the Emerging Technologies Opportunity Program to semi-automate experiments that measure microbial activity in soil.

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    A view of the mangroves from which the giant bacteria were sampled in Guadeloupe. (Hugo Bret)
    Giant Bacteria Found in Guadeloupe Mangroves Challenge Traditional Concepts
    Harnessing JGI and Berkeley Lab resources, researchers characterized a giant - 5,000 times bigger than most bacteria - filamentous bacterium discovered in the Caribbean mangroves.

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    In their approved proposal, Frederick Colwell of Oregon State University and colleagues are interested in the microbial communities that live on Alaska’s glacially dominated Copper River Delta. They’re looking at how the microbes in these high latitude wetlands, such as the Copper River Delta wetland pond shown here, cycle carbon. (Courtesy of Rick Colwell)
    Monitoring Inter-Organism Interactions Within Ecosystems
    Many of the proposals approved through JGI's annual Community Science Program call focus on harnessing genomics to developing sustainable resources for biofuels and bioproducts.

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    Coloring the water, the algae Phaeocystis blooms off the side of the sampling vessel, Polarstern, in the temperate region of the North Atlantic. (Katrin Schmidt)
    Climate Change Threatens Base of Polar Oceans’ Bountiful Food Webs
    As warm-adapted microbes edge polewards, they’d oust resident tiny algae. It's a trend that threatens to destabilize the delicate marine food web and change the oceans as we know them.

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Home › Items tagged with: microbial genomics

Content Tagged "microbial genomics"

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March 16, 2011

Roberts Wesleyan College part of DOE JGI Undergraduate Research Program in Microbial Genome Annotation

The U.S. Department of Energy Joint Genome Institute (JGI) has chosen Dr. David Roll, professor of biological chemistry and director of Forensic Science at Roberts Wesleyan College, as one of 18 collaborators nationwide to join its Undergraduate Research Program in Microbial Genome Annotation, the College announced today. Read more at the Roberts Wesleyan College news… [Read More]

March 13, 2011

Phil Hugenholtz on metagenomics and ecogenomics in Australian Life Scientist

In research published in Nature in 2007, Hugenholtz, along with collaborators from the California Institute of Technology and Diversa (now Verenium) Corporation, used metagenomics to detail the process by which a dry wood feeding termite, a Nasutitermes species, breaks down cellulose.  They generated 62 million base pairs – a “drop in the ocean by today’s… [Read More]

March 8, 2011

UCLA team makes isobutanol from cellulose on ScienceDaily

While there were many possible microbial candidates, the research team ultimately chose Clostridium cellulolyticum, which was originally isolated from decayed grass. The researchers noted that their strategy exploits the host’s natural cellulolytic activity and the amino acid biosynthetic pathway and diverts its intermediates to produce higher alcohol than ethanol. The researchers also noted that Clostridium… [Read More]

March 8, 2011

Around this time, Dey-Rao suggested that the department become involved in educational programs offered through the Department of Energy’s Joint Genome Institute (JGI). In looking into these programs, Kostyniak concluded that they would be a good fit for university-level courses, as well as for high school courses aimed at stimulating students’ interest in science careers…. [Read More]

https://jgi.doe.gov/310/

March 4, 2011

DOE JGI’s microbial work and IMG on ESNet’s Network Matters

Microbes are single-cell organisms that live in colonies and can be found in nearly every corner of our planet, in places ranging from insects’ intestines to some of the most toxic chemical environments. The site for the most detailed information on the genetic makeup of these organisms only lives in one place – at the… [Read More]

January 31, 2011

Cow rumen metagenome study in EarthSky

Enter: the cow. If cows are good at anything, it’s digesting plant material until it turns into sugar; Dr. Rubin noted that cows have been eating grass for a few million years. That’s why Rubin’s team decided to do major genetic analysis of microbes inside the stomachs of cows. He explained that he was interested… [Read More]

January 28, 2011

Cow rumen metagenome study on BBC World Service: Science in Action

Growing crops to make bio-fuel is controversial – they can take up valuable land and resources that could be used for food production and in the case of oil palms, large tracts of rainforest have been cleared to make way for this cash crop. But the second generation of bio-fuels hope to make use of… [Read More]

January 28, 2011

Cow rumen metagenome study on Mother Nature Network

Ethanol makers will produce about 13 billion gallons of the renewable fuel this year, chiefly from corn. A 2007 law requires annual use of 36 billion gallons from 2022 and reserves 21 billion gallons of it for “advanced” biofuels.   The U.S. government offered $1.5 billion in October to help bring next-generation biofuels to market…. [Read More]

January 28, 2011

Cow rumen metagenome study on GenomeWeb’s The Daily Scan

In Science this week, a team led by investigators at the US Department of Energy Joint Genome Institute reports its discovery of 27,755 putative carbohydrate-active genes among cow rumen microbes through metagenomic sequencing. The team also found that these genes “expressed 90 candidate proteins, of which 57 percent were enzymatically active against cellulosic substrates,” which… [Read More]

January 28, 2011

Cow rumen metagenome study on ABC Science

In this case, the goal was to find microbes that make enzymes that can efficiently break down the toughest fibres in switchgrass, a tough crop that can be used to produce ethanol and which can grow in places where food crops do not grow well. But switchgrass is very tough to break down. Read more… [Read More]
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