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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… the  genomes of microorganisms is incredibly useful for understanding the microbiome of the earth, but most … shotgun metagenomic sequencing and single-cell sequencing. For years, extracting single cells and then amplifying their … Amplification to generate enough DNA to put on a sequencer. As previously mentioned, it’s a method that’s …
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From Stress to Growth: The adaptive mechanisms of algae in changing Arctic waters
… manage the growth, yield and climate resilience of algae — for potential use as biomass or in biomass conversion to … thrives in the Arctic, and how different types of microbes form communities. As temperatures fluctuate, salt levels … annotated algal genome is available on the JGI’s PhycoCosm data portal.) This research not only sheds light on the …
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Honey Bee Gut Microbiota Divvy Up Dinner
… in the next generation of flowering vegetation. In return for their services, bees eat plant nectar and pollen. Nectar … carries out its helpful metabolism: who’s responsible for what biochemical processes? Now, an international team … Bifidobacterium  are able to carry out this metabolic feat. For both clades, then, strains are variable in their …
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Notes on the Microbial Diversity of Puerto Nuevo’s Coastline
… Puerto Nuevo is a small town along the Baja California coastline in Mexico. While conducting early field … waters and sediment with collaborators at the Center for Scientific Research and Higher Education at Ensenada … makes it easy for someone in my position and demographics to ask questions and learn, serving a strong role …
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JGI on the 2021 Highly Cited Researchers List
… JGI! They are among those recognized by Clarivate Analytics for demonstrating “significant influence in their chosen … Jeremy Schmutz Susannah Tringe Tanja Woyke Below are a few projects each of these researchers were involved in, … multi-institutional research team that combined field data with a reference switchgrass genome to associate …
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JGI@25: Following Fungi that Pry Apart Plant Polymers
… a chemical and biological engineer at the University of California Santa Barbara. So plants don’t break down without a … that biomass could serve as the starting material for a wide range of fuels and chemicals. To get to that … into biochemical production.    Over time, as these projects have delivered results, they have also snowballed …
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JGI Accepts 17 Proposals for Community Science Program
… on harnessing genomics to developing sustainable resources for biofuels and bioproducts. Andrew Nelson of the Boyce … tolerant shrub. Melissa Roth of the University of California, Berkeley aims to redesign and engineer algae by … collaboration that produced the reference genome sequence for Eucalyptus grandis. Accessing recently burned …
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Functional analysis of Salix purpurea genes support roles for ARR17 and GATA15 as master regulators of sex determination
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Tracking the Origins of Methane-Producing Microbes
… cycle. All methanogenic archaea use one of three pathways for methanogenesis: hydrogen-dependent and CO2-reducing, or … acetoclastic. All three pathways require the gene cluster for methane production known as Mcr. Until recently, the Mcr … the microbes into small pools of cells that were then sequenced separately. From these samples, 111 …
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Iron-rich Microbial Mats’ Main Players: Marsarchaeota
… microbial mats at Yellowstone offer researchers an analog for studying early Earth conditions. Additionally, … thousands to millions of copies of a particular DNA sequence from just a few pieces of DNA. Summary The extreme … a wide range of temperatures, levels of acidity and geographical features, and the archaeal lineages found there …
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JGI-Enabled Research Demonstrates the Existence of New Methane-Makers
… New studies show methanogenesis is not exclusively for Euryarchaeota. Atmospheric levels of methane, a potent … called methanogenesis. Methanogenic microorganisms therefore play a critical role in the global carbon cycle, and are of significant interest for their potential contributions to a sustainable …
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A Pinch of Salt Activates Silent Gene Clusters in Streptomyces
… This single environmental trigger highlights efficiency for users requiring multiple omics methods to examine … offers a template — integrative analysis of multiple omics data from various environments — that JGI users can leverage … The team also identified previously unknown promoter sequences within these gene clusters, offering new …
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