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Getting to the Bottom of Fungal Functions Across Earth’s Forests
… interactions to untangle. Fungi work in cooperation to support a forest, and species vary across Earth’s … understanding of multiple forest systems, this work sets up protocols and pipelines that other teams can use around … Saprotrophs and mycorrhizal symbionts have historically been divided into separate ecological niches — recyclers and …
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Frequently Asked Questions: Project Management
… the “ edit contacts ” page in your account. Use the form to update all information except email address. To update your … the past.  These protocols are not endorsed by and have not been validated by the JGI, but are provided as general … … Can I request a culture/slant/DNA of an organism that has been sequenced at the JGI? … How do I check the current …
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Micro-Scale Applications Group
The Micro-Scale Applications group explores the genetic makeup of natural microbial communities using a variety of cutting-edge approaches.  Our group also seeks out new approaches that utilize …
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Products
… the JGI product catalog. As a result certain products have been  discontinued , including smRNA, bisulfite sequencing, … in various combinations and cloned into a vector of choice; up to 210,000 variants can be made Pool of variant … (single organism). Note: smRNA sequencing is no longer supported. For annotation: de novo assembly. For counting: …
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User Support
… ). Once users are notified that their proposal has been accepted, the PMO will assist with initiation of work … Portals Coordinate data analysis tool tutorials, as needed Upon completion of all work associated with your proposal, …
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CSP Large Scale Call
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Igor Grigoriev
… in the JGI MycoCosm portal. In 2018, he launched scale-up of algal genomics and PhycoCosm portal. Dr. Grigoriev … Acids Res. 49(D1):D1004-D1011 Swift et al (2021) Anaerobic gut fungi are an untapped reservoir of natural products. … Grigoriev et al (2014) MycoCosm portal: gearing up for 1000 fungal genomes.  Nucleic Acids Res. …
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Supercharging SIP in the Fungal Hyphosphere
… live in complex communities, like within soils or the gut, they are difficult to study individually. Many … microbial DNA is manually separated into a few different groups based on its density — DNA containing isotopes is … and sequencing steps. Ultimately, researchers found their updated strategy required one-sixth the labor of the manual …
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Strain Engineering
The focus of this group, in recent years, has been to bring generalizable … Team    … For further details, please reach out to the Group Lead …
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Special Initiatives & Programs
… provides sequencing, synthesis, and targeted metabolomics support for DOE-BER funded awards, including awards funded … the BER funding call, proposals are NOT eligible for BERSS support through the JGI (with the exception of … call .  Process Proposals may be submitted at any time. Support is limited to work included in the BER-funded …
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Jie Guo
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Better Genome Editing for Bioenergy
… could be used for renewable fuel. In fact, lipids can make up more than 90 percent of the microbe’s dry weight. In … these gobs of energy, a team of researchers sought to disrupt every gene in the genome with CRISPR-Cas9 in order to … that could be applied to other eukaryotes. … This work was supported by DOE Joint Genome Institute grant  CSP-503076 …
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