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Tracking the Origins of Methane-Producing Microbes
… methanogenic archaea use one of three pathways for methanogenesis: hydrogen-dependent and CO2-reducing, or … mini-metagenomics to these samples by sorting the microbes into small pools of cells that were then sequenced separately. From these samples, 111 … and cleanup. The JGI provides integrated high-throughput sequencing and computational analysis that enable …
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iPHoP: A Matchmaker for Phages and their Hosts
… any host will do. It must be a specific host the virus has adapted to commandeer. For bacteriophage viruses, these hosts … are microbes like bacteria, not humans. With metagenomic sequencing, researchers have found more of these viruses … iPHoP program brings several of these approaches together into the same workflow, then uses a machine-learning model …
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JGI@25: From Life at the Extremes to Editing Genomes
… refine and improve the process of finding novel Cas genes and CRISPR systems. The collaboration has resulted in … can target single-stranded DNA. Such proteins have been adapted for therapeutic application development by Mammoth … Banfield. (Banfield’s recounting of that first – and subsequent – meetings is here .) Working with the JGI, Banfield …
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JGI@25 RECAP: 2022 Annual Meeting features historical perspectives on JGI’s 25 years
… look back at the JGI’s history, from its beginnings in the sequencing for the Human Genome Project out to the future of … he admitted that boiling that portfolio’s impact down into a succinct message was quite a challenge. “The default … not so unexpected given JGI’s remarkable ability to adapt to a changing scientific and technological landscape.” …
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A framework genetic map for Miscanthus sinensis from RNAseq-based markers shows recent tetraploidy
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Genetic dissection of natural variation in oilseed traits of camelina by whole‐genome resequencing and QTL mapping
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Expanding Virophage Diversity
… viruses. Almost all known virophage genomes share only four genes in common: major and minor capsid proteins (MCP and … increased the number of known high quality virophage genome sequences 10-fold through computational approaches. By … human (for the first time) gut and taxonomically classified into 27 distinct clades (17 of them without previously known …
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Cultivating Symbiotic Antarctic Microbes
… biosphere), including the global carbon cycle. The JGI is sequencing large numbers of microbes and microbial … that contribute to carbon cycling, and is interested in understanding the genomic basis and ecological consequences … been able to gain further insights about how microbes have adapted to thrive in polar ecosystems. The work by …
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Igor Grigoriev
… Russia PhD in Molecular Biology, Research Institute for Genetics and Selection of Industrial Organisms, Moscow, … 7(9):1419-1430. Ye et al (2022) The role of zinc in the adaptive evolution of polar phytoplankton.  Nature Ecol Evol … Miyauchi et al (2020) Large-scale genome sequencing of mycorrhizal fungi provides insights into the …
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JGI@25: Cow Rumen and the Early Days of Metagenomics
… environmental samples — to identify over 27,000 microbial genes. These genes were identified based on their potential … vital to facilitate the assembly of small DNA fragments into larger and coherent pieces called contigs — into genes … of rumen-digested switchgrass, researchers isolated and sequenced DNA from rumen microbes involved in the digestion …
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Publication Policy
… acknowledgment (see Auspice Statement below) for the generation of sequence or metabolomic data, DNA synthesized, and any other … many staff are involved in processing samples and data generation according to SOPs.  For each individual user …
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Climate Change Threatens Base of Polar Oceans’ Bountiful Food Webs
… As warm-adapted microbes edge polewards, they’d oust resident tiny … than previously suspected. “That would cause significant consequences on the entire food web, and therefore ecosystem … research cruises, they dipped their self-closing containers into sea water to sample algal communities along transects …
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