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Genomic analysis of 94 SEMIA strains isolated from common bean nodules: species diversity and genes related to nodulation, nitrogen fixation, and tryptophan metabolism
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Boosting Small Molecule Production in Super “Soup”
… extracts provide an alternative format for chemical synthesis in the absence of cell growth by isolating the soluble … strains. (Blake Rasor) Think of cells as tiny factories: within their walls they have both the machinery to make … the yeasts received fine-tuning of gene expression through genetic rewiring with CRISPR-Cas9 that enhanced their …
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A Grass Model to Help Improve Giant Miscanthus
… Miscanthus  sequence offers insights into benefits of … numerous wild Miscanthus lineages to better understand the genetic diversity of the grass, which could help in optimizing … This work was supported by the Energy Biosciences Institute … for Advanced Bioenergy and Bioproducts Innovation, which is supported by the U.S. Department of Energy, Office of …
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Extracting the Secrets of Secondary Metabolites
… CRAGE-CRISPR combo helps further demystify biosynthetic gene … is a technique researchers can use to integrate large genetic payloads directly into diverse microbes. Combining … to identify strains able to produce secondary metabolites within a lab environment, shining a light into this …
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A Pinch of Salt Activates Silent Gene Clusters in Streptomyces
… This single environmental trigger highlights efficiency for … Streptomyces are nature's chemical factories. They hold genetic blueprints for dozens of complex compounds, but stay … team also identified previously unknown promoter sequences within these gene clusters, offering new regulatory targets …
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Busting the Unbreakable Lignin
… yield fuels, and removing lignin in order to do so is a resource-intensive process. Now, a new study shows how … production and bioproduct engineering. Summary That organisms make use of cellulose and hemicellulose is … anaerobic fungi (Neocallimastix californiae) on sorghum, switchgrass and poplar, then monitored changes to the …
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Katherine Louie
… offered by the platform are a result of Dr. Louie’s establishment of a strong core foundation of robust mass … workflows for polar and nonpolar metabolite analysis, creation of large metabolite databases by running 1000s … DNA-SIP for helping identify active micro-organisms within complex communities, and custom software for …
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Uncovering Novel Genomes from Earth’s Microbiomes
… Large-scale comparative analysis leads to identification of biosynthetic gene clusters for … genomes for just a small fraction of Earth’s microbial diversity. Because most microbes cannot be cultivated under … it’s striking how many uncultivated lineages are only represented by MAGs,” he said. “While these draft genomes are …
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Webinar: SIP technologies at EMSL and JGI
… provide access to a variety of tools that leverage stable isotopes to measure carbon and nutrient flux, and identify … the isotopomers. We estimate the overall atom enrichment within the bulk community DNA, and that gives us a rough … is the LA-IRMS for tracking N15? The example in the presentation was with C13.  A:  Yes, EMSL can look at N15 as …
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JGI@25: The Little Alga That Could
… In honor of the JGI's 25th anniversary in 2022, we have revisited a number of notable achievements that showcase our … sequences have been cited in almost one-fourth (23.8%) of publications focused on that specific algae. The reference … lab and second author on the paper, studied viral genetics and gene therapy before self-training himself as a …
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A Cost-Efficient Way to Convert Yeast into a Cell Factory for Hydroxyapatite
… Innovative technology from this biomanufacturing collaboration is available for … to recover high value products from human waste. They   genetically modified yeast to efficiently convert elements present in urine to create hydroxyapatite, a high-value … This work was supported by laboratory-directed research and …
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Ray Turner Completes Last Tour of Lab Duty at JGI
… as JGI’s Operations Deputy has come to a close as this previously retired Naval Commander retires from Berkeley … person needs establish the culture of workforce safety and diversity and working together and making a welcoming and … are 100 percent dependable. Your ethics always point due north.” Joe Harkins served as project lead on the new …
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