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Better Genome Editing for Bioenergy
… A team has optimized a crucial part of CRISPR-Cas9 technology to enable improvements in … tool will make the right cut, researchers created a set of guide RNAs that were effective against 94 percent of the … sgRNA sequences: six for every gene in the genome. The JGI  DNA Synthesis Science team synthesized these sgRNAs, cloning …
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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… from single cells. The Science Studying the  genomes of microorganisms is incredibly useful for understanding the microbiome of the earth, but most species do not grow easily in the … years, extracting single cells and then amplifying their DNA for genome sequencing was no easy task. The traditional …
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Celebrating a Decade of Science through the JGI-UC Merced Genomics Internship Program
… 2023 marked a decade since the inception of the flagship internship program between the DOE Joint … UC Merced Senior Assistant Dean and CFO of the School of Natural Sciences Ernie Costello beamed as he spoke of what … working with Elizabeth Dumont , Dean of the School of Natural Sciences, to make sure that they provide the …
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New and Improved: Eucalyptus Reference is Now Accessible
… a new reference genome for eucalyptus in the July edition of the journal G3 Genes | Genomes | Genetics , with … Africa. The South African team collected the plant tissues. DNA and RNA sequencing were conducted by the JGI, along with … the researchers glimpses of the population and pangenome variations in the eucalyptus species. The researchers are …
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Getting to the Bottom of Fungal Functions Across Earth’s Forests
… registers the sound — one thing is for sure: there are lots of fungi around. Within a forest’s soil, hundreds of species decompose … who nominated species for sequencing and then sent DNA and RNA samples to JGI,” Grigoriev said. All of these … The U.S. Department of Energy Joint Genome Institute, a DOE Office of Science …
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Expanding Virophage Diversity
… Virophages are small viruses with double-stranded DNA genomes that co-infect eukaryotic cells along with giant … in  Microbiome , researchers from the US Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science facility, have increased the number of …
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A Cost-Efficient Way to Convert Yeast into a Cell Factory for Hydroxyapatite
… hydroxyapatite, a high-value biomaterial with a wide range of industrial applications. The team was led by researchers at two U.S. Department of Energy national user facilities – the DOE Joint Genome … realized that the yeast   Saccharomyces boulardii  was naturally performing activities similar to the specialized …
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JGI Accepts 17 Proposals for Community Science Program
… Monitoring Inter-Organism Interactions Within Ecosystems Latest portfolio of approved proposals highlight biodiversity and how ecosystems are adapting to stresses. A total of 17 proposals have been approved through the annual …
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JGI Early Career Researchers Featured in mSystems Special Issue
… In a special issue of mSystems , out May 14, 2019, JGI researchers are among the authors who offer perspectives on what the next five years of innovation … in soil for the first time. A second approach known as DNA stable isotope probing (DNA SIP) relies on incubating …
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A More Intuitive Phytozome Interface
… Highlighting the latest interactive version of the JGI’s plant data portal. On August 6, 2021,  version 12 of Phytozome will be retired, and  version 13 will become … (for viewing and modifying multiple sequence alignments). Variation view on individual gene pages The ability to …
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Thawing permafrost, microbes, and viruses: Gary Trubl — Part 1
… wants to understand how viruses impact the release of greenhouse gases from Arctic permafrost. But to see what they’re doing, he first needs their DNA.   …
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High Five: Celebrating the JGI-UC Merced Genomics Internship Program
… than 20 UC Merced students have contributed to the research of 13 JGI scientists since the program’s inception. In 2014, … Merced assistant professor Suzanne Sindi from the School of Natural Sciences and started a program that offered a … message from Betsy Dumont , Dean of the UC Merced School of Natural Sciences in honor of the occasion. To a packed …
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