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… already had a fermentation process with already engineered strains, but this was to further build on those to try and … and so my team, we were responsible for coming up with new strains and new process improvements and new ways to recover … to some people more about down the road. Email Nigel! He’ll sequence whatever you want!  [LAUGHTER]  NIGEL MOUNCEY: We …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … get made fun of by my now-wife, because she was wondering why it is that we needed to produce more toxins in the lab …

DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … – how bacteria or other organisms communicate and how and why secondary metabolites are being produced in the … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

… in the analysis and publication of the claimed genome sequences. This opportunity, provided by the Department of … builds upon thousands of microbial genomes that have been sequenced by the JGI though not formally analyzed or … necessary to extract meaningful biological insights from sequence data, employing IMG data and tools for comparative …

… out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk … this and that. They’re doing all this 16S and I said, “Why don’t you just include the ITS too? It’s so easy to add …

… analysis of thousands of archaeal and bacterial type strains ( GEBA-type strains project ), the delineation of … al. (2017) Protein structure determination using metagenome sequence data. Science   355(6322):294-298 Paez-Espino D. et … al. (2015) Microbial species delineation using whole genome sequences. Nucleic Acids Res. 43(14):6761-71 Kyrpides NC et …

… and eukaryotic microbes and viruses in environmental sequence data. We use multi-omics (metagenomics, …

… through end-to-end automation, and further advancing sequence applications, single cell genomics, DNA synthesis, …

… tool BOOST , which provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … favorite examples of a secondary metabolite producer, and why they produce those metabolites? MARC: Well, I’m really …

… able to explore DNA sequencing and actually get to the DNA sequence – sequences for the genes that code for the … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less … biology perspective, like if you have a cluster of genes, why can’t you just engineer you know, a promoter or an …

… and Environmental Research (BER) program, set the final sequence allocation for this program. …