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… – problems that might need special technology for DNA sequencing, genomics, metabolomics, and synthetic biology, … stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … the JGI. And so now here at the JGI, I work with all of the sequencing data that the JGI produces to try to find and …

… environmental samples. This work combines third generation sequencing and machine learning to enable fast, accurate … A read simulation tool for generating synthetic sequencing data from genomic sequences …

… For accepted proposals, sequencing, synthesis, metabolomics and analysis will be … with DOE mission-relevant projects that call for expansive sequencing, synthetic biology, and metabolomics capabilities … Letters of Intent. Proposals may include synthetic biology, sequencing, and metabolomics. Proposals may include …

… resources empower students through isolation and genome sequencing of new fungal species.  Students collect fungi, … into iNaturalist. Myco-Ed has:  ~50 lineages selected for sequencing 16 genomes released 20 members from 15 … laboratories with minimal overhead (cost of media, ITS sequencing and tissue preparation), allowing participation …

… community at large with access to high-throughput sequencing, synthesis, and metabolomics at the JGI for … based on their DOE relevance.  For accepted proposals, sequencing, synthesis, metabolomics and analysis will be …

… the Broad that was looking to develop new methods for DNA sequencing of microbes. And what kept breaking our methods … So you were saying, Mark, how the DNA that was breaking the sequencing from really being functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways …

… your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … was jumping onto the I ntegrated Microbial Genomes and Metagenomes, the IMG platform that JGI has that really got … JGI and this was before they were integrated. So I thought, why not feed it in there. And I went to group meeting and …

… to only three phyla. The Woyke group uses single-cell sequencing as a means to begin filling this gap. A primary … genomes are critical to serve as phylogenetic anchors for metagenomes studies.  While uncovering the metabolic … of interest, prior to and in conjunction with whole genome sequencing. …

… in an obvious way. NIGEL MOUNCEY: We did a little bit of metagenome screening work and did find related clusters. But … NIGEL MOUNCEY: Oh, sure. I think our legacy obviously is in sequencing.  DAN: Of course.  NIGEL MOUNCEY: Sequencing the wazoo out of anything that’s relevant to the …

… Download plant, algal, fungal and microbial genomes and metagenomes — top-quality genomic data, open to all … comprehensive access to information regarding genome and metagenome sequencing projects, and their associated metadata, around … capability of microbial communities based on their metagenome sequence, in the context of reference isolate …

… datasets encompassing >150,000 isolate genomes, 230,000 metagenome-assembled genomes (MAGs), and 40,000+ metagenomes along with curated environmental- and other …

… DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … is similar to primary metabolism. So if you were, you know, sequencing an organism for the first time, and you’ve never … more knowledge you can generate. So that’s why we are still sequencing lots of stuff, and why the JGI is a key driver in …