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Expanding Virophage Diversity
… in  Microbiome , researchers from the US Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office … increased the number of known high quality virophage genome sequences 10-fold through computational approaches. By … 28,000 unique MCP sequences.  Further analysis led to the identification of 328 “high quality” (based on completeness) …
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Shattering Expectations: Novel Seed Dispersal Gene Found in Green Millet
… Survey of Setaria's natural diversity reveals seed dispersal in … National Laboratory (Berkeley Lab), generated genome sequences for nearly 600 green millet plants and released a very high quality reference S. viridis genome sequence. Analysis of these plant genome sequences also led …
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Tracing the Evolution of Shiitake Mushrooms
… New genomes highlight the diversity of white rot fungi in the genus Lentinula   The Science … at Lentinula samples from around the world. By complete sequencing of 24 new mushroom genomes, and assembling … that Lentinula fungi rely on to decay wood were widely conserved across species. Specifically, many species had …
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Marchantia Pangenome Highlights Adaptive Nature of Plant Lineages
… Understanding the shared genetic toolkit of ancestral and present-day plants The Science In a recent  … plants, or angiosperms, suggesting they are evolutionarily conserved across plant lineages. They hypothesized that … framework provided by these high quality reference genome sequences could help researchers better understand related …
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An Enzyme Family that Helped Shape Nitrogen Metabolism on Our Planet
… characteristics — creating many different ways of processing nitrogen. To boost crops more efficiently in … — they rely on enzymes that have been evolving for billions of years. However, these enzymes are often loosely … 62 distinct AT groups. However, no single AT group was conserved across all species — a surprise for an enzyme that …
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JGI@25: A Single Cell, Myriad Microbial Discoveries
… In honor of the JGI's 25th anniversary in 2022, we have revisited a … cell. Several years later, the continuing single cell sequencing collaboration between the JGI and Bigelow Lab led to the identification of key microbial culprits in carbon fixation …
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A Pinch of Salt Activates Silent Gene Clusters in Streptomyces
… chemical factories. They hold genetic blueprints for dozens of complex compounds, but stay largely dormant under … salt triggers this metabolic shift, the team combined RNA sequencing across multiple time points with Cappable-seq, a … efforts. Importantly, several of the activated pathways are conserved across hundreds of Streptomyces species, pointing …
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Doubling Down on Known Protein Families
… online Oct. 11, 2023 in  Nature highlights the vast array of functional diversity of microbes through a novel approach … replicating a whole community is impossible.  Metagenomic sequencing allows researchers to study the entire genetic makeup of these communities via whole genome sequencing of the samples, without being able to distinguish …
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New Research Sheds Light on Diversity in the Deep Sea
… get any sunlight, and yet they still support a unique array of microorganisms adapted to living in extreme conditions. … five oceanic regions. In doing so, they found a high degree of variability in the types of microorganisms that inhabit … Berkeley National Laboratory, contributed metagenomic sequencing to the study as well as  genome analysis, …
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Finding the Fermenters
… researchers built an interactive and comprehensive database of fermentative prokaryotes. The Science Most organisms use … process called fermentation. To better understand the range of bacteria and archaea that rely on this form of metabolism, researchers recently compiled a list of with …
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User Programs
… For accepted proposals, sequencing, synthesis, metabolomics and analysis will be paid for by the Department of Energy’s funding of the JGI. There is no cost to the user … with DOE mission-relevant projects that call for expansive sequencing, synthetic biology, and metabolomics capabilities …
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Refining the Process of Identifying Algae Biotechnology Candidates
… well ] The Science The recipe for growing algae as a source of sustainable biofuels seems fairly simple: place these … Researchers collaborating across multiple institutions have sequenced and analyzed the genome of an alga that thrives in … which genes expression patterns. The analysis led to the identification of several transcription factor regulating …
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