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… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… is– what do you think is the thrust of your lab, going forward? Kate Duncan: Yeah, so I guess we’re really interested … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain … PARKINSON: Yeah. We definitely follow up with them afterwards. And so we don't always have the ability to find the …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a … for their contributions just to move this research forward. DAN UDWARY: One of the things in this and the …

… able to explore DNA sequencing and actually get to the DNA sequence – sequences for the genes that code for the … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less … has the opportunity to, you know, use that research towards secondary metabolism aims. And we need new people – we …

… in the analysis and publication of the claimed genome sequences. This opportunity, provided by the Department of … builds upon thousands of microbial genomes that have been sequenced by the JGI though not formally analyzed or … necessary to extract meaningful biological insights from sequence data, employing IMG data and tools for comparative …

… that they make? CASSANDRA QUAVE: Yeah, so I mean my path towards natural products and medical ethnobotany really began … culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … Medema: Yeah. So that’s an ERC grant that we recently got awarded. And this is indeed one of the new directions that my …

DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … – how bacteria or other organisms communicate and how and why secondary metabolites are being produced in the … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

Apple Podcasts or wherever you’re getting this and tell me why you’re listening. DAN: But, now, here’s Natural Prodcast … do that is still quite tricky. You can’t easily do it by forward prediction from the gene cluster. Not to the discrete … And that’s been followed by developments in pulse sequences and improvements in the ways in which you acquire …

… embargo starting at construct or strain delivery.  Detailed sequence information for constructs are made publicly …