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… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a … and you might have the same cluster and all these different strains. But only one produces the compound. So I think …

… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more … of the fun things that I hope that I can express today is why secondary metabolism is interesting and why it is that …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these … section. We want to be sure that the PIs can articulate why their work falls within one of these DOE mission areas. …

… in the analysis and publication of the claimed genome sequences. This opportunity, provided by the Joint Genome … builds upon thousands of microbial genomes that have been sequenced by the JGI though not formally analyzed or … necessary to extract meaningful biological insights from sequence data, employing IMG data and tools for comparative …

… algae, examining both natural populations and engineered strains. This approach allows for the identification of key … The  1000 Fungal Genomes Project is a five-year project to sequence 1000 fungal genomes from across the Fungal Tree of … of fungi. Mycocosm enables users to navigate across sequenced fungal genomes, and to conduct comparative and …

… Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the … And we’ve given them genome sequences of some of these strains. They’re not all Pseudonocardia . There’s other very …

… you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a … tells you what some of those design rules might be and constrains hypotheses that you can test about how to put things …

… DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … – how bacteria or other organisms communicate and how and why secondary metabolites are being produced in the … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

… out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk … this and that. They’re doing all this 16S and I said, “Why don’t you just include the ITS too? It’s so easy to add …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … get made fun of by my now-wife, because she was wondering why it is that we needed to produce more toxins in the lab …

… already had a fermentation process with already engineered strains, but this was to further build on those to try and … and so my team, we were responsible for coming up with new strains and new process improvements and new ways to recover … to some people more about down the road. Email Nigel! He’ll sequence whatever you want!  [LAUGHTER]  NIGEL MOUNCEY: We …