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JGI@25: Expanding Metagenomics to Capture Viral Diversity
… In honor of the JGI's 25th anniversary in 2022, we have revisited a … the microbes there,” said Simon Roux , the Viral Genomics Group Lead at the JGI.  To understand the … Along with highlighting the viruses in a given sample, metagenomics shed light on another key aspect of viruses in the …
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JGI User Executive Committee Expands
… have been elected to the User Executive Committee (UEC) of the U.S. Department of Energy Joint Genome Institute … genetics expertise and experience as a primarily fungal genomics user, he aims to help align JGI policies with … years is eligible for election to the UEC. Members serve three-year terms and can serve no more than two consecutive …
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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
… ( CRAGE ). CRAGE enables scientists to insert large pieces of DNA (up to 60 kb) in a single step, directly into the genome. “CRAGE is central to our ability to offer host-engineering for our users,” says JGI Director … as part of the Community Science Program (CSP)  Functional Genomics proposal call. …
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Building Wet and Dry Lab Skills
… Summer 2025 marked the second year of Alabama A&M University students coming to the DOE Joint … experience funded by a DOE training grant.  Building off the previous year’s project, undergraduate interns … mentors Yi Zhai and John Vogel from the Plant Functional Genomics Group to study the effects of  Brachypodium …
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Kjiersten Fagnan
… closely with staff to understand the data-intensive nature of JGI workloads. Dr. Fagnan was appointed CIO of the JGI in 2016, and in 2018 was hired to be the JGI’s … Methodologies workshops. In 2018, Dr. Fagnan was part of the Gordon Bell prizewinning team at the Supercomputing …
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Extracting the Secrets of Secondary Metabolites
… survival, may now be easier to characterize following a proof-of-concept study in which researchers paired CRISPR and … effectiveness has historically been limited due to the lack of robust tools available for carrying CRISPR into … The work conducted by the US Department of Energy Joint Genome Institute, a DOE Office of Science …
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Sequencing Sphagnum Leads to Discovery of Sex Chromosomes
… A nuanced understanding of peat moss drives insight into carbon storage   The Science Boggy peatlands, which hold much of the Earth’s carbon as well as material that can be converted to energy, are made up heavily of sphagnum mosses.  New research identifies sex chromosomes …
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An Addiction Crucial to a Fungus’ Reproduction
… A fungus relies on bacteria to regulate key components of its reproductive machinery The Science To better … , is recognized as a mutualist, where both species of organism benefit from association, but was predicted to … researchers also generated the first transcriptomic dataset of sexual reproduction in early fungi and discovered genes …
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A Pinch of Salt Activates Silent Gene Clusters in Streptomyces
… chemical factories. They hold genetic blueprints for dozens of complex compounds, but stay largely dormant under standard conditions. As described in a recent issue of mSystems , scientists found that adding salt to the … provides engineers concrete targets for optimization. Many of the metabolic switches identified are shared across …
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An Automated Tool for Assessing Virus Data Quality
… (metagenomes). However, the quality and completeness of metagenome-assembled virus sequences vary widely. In a … to help researchers assess and improve the quality of metagenome-assembled viral genomes . The Impact The … DNA sequencing can help scientist to recover the genomes of these viruses and associate them with their microbial …
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In Hot Spring Microbial Mat, Viruses Ride “Piggyback”
… a microbial mat. The Science In microbial mats, communities of microorganisms live among viruses that infect them. But … a more systematic picture through single-cell and metagenomic sequencing. A team co-led by Mária Džunková, … virus in 34 hosts or 26 percent of the single cells. Metagenomics sequencing then allowed the team to ask, for the 34 …
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Supercharging SIP in the Fungal Hyphosphere
… The Science Researchers have successfully automated aspects of a commonly-used process for studying microbial … with results published in the  November 2022 issue of Microbiome . High-throughput stable isotope probing (SIP) … and improve results. Applying this method to the study of a particular fungi, researchers identified novel …
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