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Waiting to Respire
… Genomes from uncultivated bacteria offers clues to ancestral … teams joined forces and data sets to describe bacterial genomes for related (“sibling”) lineages Margulisbacteria … Paula Matheus Carnevali was working with a group of metagenome-assembled genomes (MAGs) dubbed Margulisbacteria, and …
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Fishing for Novel Cellulose Degraders
… by Tanja Woyke at the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User … to sort cells into several small pools, followed by whole genome amplification and shotgun sequencing. The team first … To validate the predicted cellulases from their captured genomes, the team then used DNA synthesis and cloning, …
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Identifying the Microbial Cleanup Crew at a Superfund Site
… break down PCE into the chemical compound ethene. Applying genome sequencing and analysis, the researchers identified … samples from wells at the site in 2007, 2009, and 2010. DNA was extracted from the samples that were sequenced by the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User …
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Silver age of GOLD introduces new features
… The JGI’s Genomes OnLine Database celebrates 25 years as a vital … The Science Since its launch 25 years ago, the  Genomes OnLine Database has matured from six projects on a … carried out at the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User …
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Benjamin Cole
… interact with their environment. Prior to joining the Joint Genome Institute, his focus was primarily on how plants … temperature oscillations throughout the day. At the Joint Genome Institute, Dr. Cole’s research focus has been on …
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Cultivating Symbiotic Antarctic Microbes
… consequences of microbe-microbe interactions. Using metagenomes recovered directly from environmental samples and … dependent on the other for required nutrients—is genome size. The obligate symbiont’s genome is often reduced compared to that of a free-living …
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Plotting a Model for Virus-Host Warfare Deep Below Ground
…   Visualizing a novel, candidate viral genome found in the deep subsurface ecosystem. The Science … of countering multiple attempts at virus infections. Their genomes include short DNA sequences that code for CRISPR … researchers at the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User …
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Tracking the Origins of Methane-Producing Microbes
… Biohub, and the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science user … and Verstraetearchaeota lineages. The archaeal genome was detected in samples collected by Stephen Quake’s … were then sequenced separately. From these samples, 111 metagenome-assembled genomes (MAGs) were recovered, most of them …
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JGI@25: A Single Cell, Myriad Microbial Discoveries
… year, the JGI team reported the first closed and finished genome derived from an uncultured bacterial single cell – a … oil plume from Deepwater Horizon oil spill led to a draft genome of the first deep-sea, oil-eating bacterium from a … researchers sequenced and assembled the first complete genome of an uncultured symbiont. The single cell was …
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In Hot Spring Microbial Mat, Viruses Ride “Piggyback”
… lysogeny, they can insinuate themselves inside the host’s genome, living quietly and replicating with their host … researcher at the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User … used single-cell sequencing to sequence both a cell’s genome and detect accompanying viral sequences, which would …
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JGI@25 RECAP: 2022 Annual Meeting features historical perspectives on JGI’s 25 years
… from its beginnings in the sequencing for the Human Genome Project out to the future of the institution. Their … very first project — the revolutionary work of the human genome project, which spanned nearly two decades, was filled … along the way. In this historical perspective of the Human Genome Project , Patrinos walked the audience through the …
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Tracing the Evolution of Shiitake Mushrooms
… New genomes highlight the diversity of white rot fungi in the … around the world. By complete sequencing of 24 new mushroom genomes, and assembling genomes from 60 existing sequences, this work expands the … FEDER funds) to F.J.R.-D., S.C., and A.T.M., grant PID2019-103901GB-I00 of the Spanish Ministry of Science and …
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