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Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation
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iPHoP: A Matchmaker for Phages and their Hosts
… to commandeer. For bacteriophage viruses, these hosts are microbes like bacteria, not humans. With metagenomic sequencing, researchers have found more of these viruses … Within the domains of archaea and bacteria, millions of  microbes govern ecosystems. These organisms carry out …
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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… easily in the lab. To access the genomes of uncultured  microbes , scientists usually employ two different methods; shotgun metagenomic sequencing and single-cell sequencing. For years, extracting single cells and then …
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Identifying the Microbial Cleanup Crew at a Superfund Site
… down PCE into the chemical compound ethene. Applying genome sequencing and analysis, the researchers identified other … to bioremediation efforts for decades. Identifying resident microbes that can help bioremediate toxic substances has led … (ERD.) It is dependent on the presence of the existing microbes in the groundwater and adjusting the environmental …
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Viruses Reprogram Cells into Different Virocells
… infections cause significant metabolic changes in marine microbes, the answer is no. They refer to the infected … changes they’ve undergone. The Impact The number of microbes in, on, and around the planet is an astronomical … in one proposed research project. For this project, RNA sequencing was done by the JGI, and proteomics and …
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The Case for Conservation
The genome assemblies highlight the multiple advances in sequencing technologies applied to the effort. The Impact … comes from finally having an answer to the question, “Why bother keeping this?” For Stuart McDaniel’s lab at the … to fully sequence the fire moss genomes ran the gamut of sequencing technologies, from making a bacterial artificial …
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Supercharging SIP in the Fungal Hyphosphere
… it. The isotopes are incorporated into the DNA of specific microbes who consume the labeled food or water. Later, their … which microorganisms used the labeled substance and which microbes did not. This is normally an arduous wet lab … at a larger scale through downstream DNA purification and sequencing steps. Ultimately, researchers found their …
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Uncovering Novel Genomes from Earth’s Microbiomes
… for multiple applications.       Despite advances in sequencing technologies and computational methods in the … small fraction of Earth’s microbial diversity. Because most microbes cannot be cultivated under laboratory conditions, … individual organisms, using various methods for processing, sequencing and analysis. “Using a technique called …
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Honey Bee Gut Microbiota Divvy Up Dinner
Scientists find bee gut microbes have a division of labor when it comes to … is a division of labor by these bacterial groups. But why the strain variability? The authors think the reason may … and cleanup. The JGI provides integrated high-throughput sequencing and computational analysis that enable …
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An Integrated Dataset to Understand Ecosystem Interaction
… on the rhizosphere and interactions between the trees, microbes and soil chemistry offers insights into how these … metagenomics, scientists were able to analyze the DNA of microbes living around the tree roots in the rhizosphere. … generated is 25 terabytes in size. Resources for genomic sequencing, analysis and characterization were provided by …
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Designer DNA: JGI Helps Users Blaze New Biosynthetic Pathways
Program , the JGI enables large-scale synthesis support, sequencing, integrative -omics, and data mining, empowering … applications — are evolving. “JGI is famous for its work sequencing human and environmental genomes. But we now have … on thiolases, a superfamily of enzymes found only in microbes, which make carbon-carbon bonds. This ability is …
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Doubling Down on Known Protein Families
Nature highlights the vast array of functional diversity of microbes through a novel approach to better understand … replicating a whole community is impossible.  Metagenomic sequencing allows researchers to study the entire genetic makeup of these communities via whole genome sequencing of the samples, without being able to distinguish …
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