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… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … disparate environments. There’s some marine. There’s some soil samples. There’s human gut samples. There’s lots and …

… DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have … products because they’re easy to find. They grow in the soil. These are the same guys that produce geosmin. And so …

… by exploring plants’ role in capturing carbon, detoxifying soils, interacting with other organisms, and adapting to … of how plants have naturally adapted to varied climate and soil conditions around the globe to identify genes to … manipulation in the laboratory. Both germplasm (sequenced mutants and natural accessions) and protocols …

… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more … is a chemical compound called geosmin that’s produced by soil bacteria that live all over the place. So that smell of …

… And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … off through these areas. And you can do– with a teaspoon of soil from outside, you maybe have to do a 10,000-fold …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … DAN UDWARY: That’s weird. I hadn’t heard that before. Why is that? Why would that be the case? ALISON NARAYAN: …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … the action of the microbiome because if you sterilize the soil, the protective effect is gone. You can transplant the …

… For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …

… or online catalog, I guess. So we've also been able to get soils from Purdue campus itself and been able to actually … The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … think the terms we should be using for what we do are, and why there and why it’s important to make those distinctions. … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …