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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… preparation, DNA synthesis and pathway engineering Mass spectrometry based metabolomics Below is a table of standard … sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs …

Sample Metadata form Sample metadata is collected via spreadsheet or directly via a web interface prior to … our Taxonomy Quick Guide. PM sends PI the sample metadata spreadsheet to fill out (or provides a link to complete the … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

Following DOE approval, project managers negotiate project specifics with PIs (including JGI scientists as needed), and … … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …

… a few months ago when we were at the height of the initial spread of the pandemic in Europe. So if there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… what it can do, and as chemists take those structures as inspirations to really start making other molecules related to … enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh …

… metabolism, or what a secondary metabolite is versus what a specialized metabolite is, then this is the podcast for you. … least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… field. Hoping to do these every once in a while, especially when we start talking to people who specialize in … more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … or MoClo assemblies … Check out our organism-agnostic CRISPR gRNA design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …