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… wouldn’t it be cool to look at these– it was actually marine invertebrates there– but look at these things in the … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … quality. Yeah, for sure. NADINE ZIEMERT: Yeah, Dan… it’s a marine actinomycete bacterium. So they look kind of like … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… of Oceanography in San Diego. Bill is a pioneer of marine natural products, getting into the ocean and looking … learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

… grade list of goals in life: on that list are being a marine biologist and being a chemist, and it’s actually … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… working with him.  We talk about his father, the legendary marine natural products chemist, Richard Moore, and get into … then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against …

When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … come from all kinds of disparate environments. There’s some marine. There’s some soil samples. There’s human gut …

… and the JGI user community below for DNA preparation tools, high molecular-weight DNA extraction and RNA preparation.  … can be found here . DNA Extraction Protocols: … Tips for High Molecular Weight DNA extraction: … RNA Extraction …

… prefers to be known among friends in natural products. He’s from Langebio in Irapuato, Mexico, and an incredibly bright … least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

QC Protocol DNA Extraction 101 : tips, kits, and protocols (from PacBio) Guidance for sample collection and storage (from PacBio) Best practices for High Molecular Weight DNA extraction and quality control … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… the product and deliverables as well as target cycle times from sample receipt to completion of standard analysis.  Raw … sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … ( IMG ) 280/298 Microbial Improved Draft, Isolate High quality draft assembly of long-read sequence data, …