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Protocols
Find protocols provided by JGI and the JGI user community below … DNA extraction and RNA preparation.  JGI sample QC protocols can be found here . DNA Extraction Protocols: … Tips for High Molecular Weight DNA extraction: …
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How to Submit Sequencing Samples
… of the process needed to prepare and ship samples for sequencing by the JGI.  Additionally it includes … . … 1) Review JGI sample QC requirements and protocols Obtaining DNA and RNA of suitable quantity and … material is one of the greatest predictors of a successful sequencing project. It is imperative that users properly …
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Partnerships: Myco-Ed
… resources empower students through isolation and genome sequencing of new fungal species.  Students collect fungi, … into iNaturalist. Myco-Ed has:  ~50 lineages selected for sequencing 16 genomes released 20 members from 15 … 300+ students 800+ iNaturalist observations All MycoEd protocols, course materials and bioinformatics exercises are …
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Sequencing Technologies
The Sequencing Technologies Group applies cutting edge molecular capabilities and state-of-the-art next-generation sequencing to enable biological discovery by the JGI and external DOE users. Adopting the latest  sequencing technologies has allowed the JGI to increase …
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Sequencing Submission Requirements for DNA Synthesis
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Frequently Asked Questions: Project Management
… manager may then schedule and allocate resources for DNA sequencing. … No. However, the JGI does maintain a list of protocols, found in our HERE (TK) , used successfully by … … Guidance on international shipments can be found in the Sequencing Sample Overview file in our HERE (TK) .  Note: …
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Institutional User Agreements
… any work can begin, including the submission of samples for sequencing, or the submission of sequencing data for synthesis. This agreement, required by … for Federal Agencies , DNA Synthesis Appendix  and DNA Sequencing Appendix are available for download.)  After your …
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DOE Metrics
FY 2025 DOE Metrics Sequencing Quarter Total Bases (Trillions) Operating Hours …     Q4 2025 175     2,167     *Includes Illumina and PacBio sequencing platforms. **Operating Hours target is based on … even though an individual may have several projects/roles. Sequencing Quarter Total Bases (Trillions) Operating Hours …
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Products
The JGI has diverse capabilities in: DNA and RNA sequencing, Sample and library preparation, DNA synthesis … of standard analysis.  Raw data is provided for all sequencing products below. In addition, JGI will submit raw … have been  discontinued , including smRNA, bisulfite sequencing, and ChIP-seq. … Scientific Program Product …
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Natural Prodcast Episode 15: Katherine Duncan
… before– or kind of around about when the first genome sequencing was done. But natural products– marine natural … around in natural products for a lot longer than genome sequencing, when we do a mass spec– a mass spec is like a … just ties in with what I just said, this was the go-to for protocols for how to do things, how to work with these …
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Partnerships: Adopt-A-Genome
… of submitting, getting feedback , and all the rules and protocols that go along with submitting a paper. That was …
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Frequently Asked Questions: Proposal Submission
… contact us in advance for advice. … For accepted proposals, sequencing, synthesis, metabolomics and analysis will be … Energy’s funding of the JGI. … No, the proposal is only for sequencing, metabolomics and/or synthesis effort at the JGI. … in your proposal. … While, in general, requests will be for sequencing and its analysis, investigators are encouraged to …
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