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… of secondary metabolism. Welcome back. This will be episode 12 of Natural Prodcast. And it’s our conversation with … Europe. So if there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And … in the early 2000s? Late 2000s? NADINE ZIEMERT: No, yeah, 2010. ALISON TAKEMURA: 2010, OK. You’re a young faculty. …

… the Molecular Foundry, the project needs to be scoped for 12 months or less. We can help you scope that part of the proposal. Also, once awarded, users have 12 months before they need to activate their project. … end of an active proposal in order to continue access in 12-month increments.  At the JGI, a typical proposal is …

… as well as a unique opportunity to complete a California K-12 science or math teaching credential as an undergraduate …

… in Yu et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 1253–1262, 70:3 ALISON TAKEMURA: And so did you succeed in … Microbiomes” paper is here . Read the associated JGI news release here . Software we discussed, used for genome … reviews of genome mining: Ziemert, Alanjary, and Weber’s 2016 overview of genome mining (open access) The …

… there a way to do that? DAN UDWARY: Well, so there’s good news and bad news on that. So one of the things that we’re trying to do … happening. And so we should have somewhere around 10, 11, 12 million BGCs in the database on the release, and that’s …

… annotate, and bin viruses from metagenomes. mSystems 9:e00888-24. https://doi.org/10.1128/msystems.00888-24 Coclet, C., Sorensen, P.O., Karaoz, U. … ecosystem. Microbiome 11, 237. https://doi.org/10.1186/s40168-023-01666-z Neri U., Wolf, Y., Roux S., Camargo A.P., …

… right? BEN: Yeah, yeah. Well, we are at [10 to the] minus 12 [Molar concentration]- is sufficient to kill cells. So … So I wanted to ask you also about – because you were in the news recently – TSRI has acquired the Pfizer strain … is a geographic distribution. So they were collected from 108 countries and regions. So, this is really collected from …

… like a nice summer day in Seattle I think this was like 2013 or 2014. And antiSMASH — I know you’ve interviewed … access to the whole sigma catalog of precursors in their 12C form. You can feed those and look for incorporation in … would grow up our bacteria on 13C methane or methanol, feed 12C methionine, and we were able to pull out the quorum …

… 10 to the negative 9th. Picomolar, 10 to the negative 12th. DAN: Yeah. So very-- BETSY PARKINSON: Very, very-- … what was really interesting is when I started my lab in 2018, several papers came out right in a row all about this … the issues is in order to make the tetrapeptide, that's a 12 membered ring. And there's extreme ring strain in 12 …