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… of secondary metabolism. Welcome back. This will be episode 12 of Natural Prodcast. And it’s our conversation with … Europe. So if there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And … in the early 2000s? Late 2000s? NADINE ZIEMERT: No, yeah, 2010. ALISON TAKEMURA: 2010, OK. You’re a young faculty. …

… getting them out to you all. But for now, here is episode 10 of Natural Prodcast, the primer on genome mining. Hey, … in Yu et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 1253–1262, 70:3 ALISON TAKEMURA: And so did you succeed in … Microbiomes” paper is here . Read the associated JGI news release here . Software we discussed, used for genome …

… so there are– I hesitate to make a guess but maybe 50 to 100 different strains and they are in individual tubules.  … in the field, these glacial bacteria grow slow and it’s not 100% clear that we know what their biological activities …

Adopt-a-Genome has:  11 CSU educators working with the JGI 120 genomes claimed (or about one per student) Seven … Xenorhabdus cabanillasii DSM17905, Xenorhabdus ehlersii DSM16337, Xenorhabdus japonica DSM16522 R Robertson, et. al.  2023 . Microbiology Resource Announcements. 2023 12 (10), e00548-23 Whole genome sequence of the denitrifying …

… metagenomics. Nature Reviews Microbiology volume 20, 721–736 JC Villada, F Schulz (2022) The endosymbiotic box of … components. Science, 356 (6333), 82-85 Schulz et al. (2016) A Rickettsiales symbiont of amoebae with ancient … in the Gammaproteobacteria. Scientific reports. 5 (1), 1-10 Schulz et al. (2014) Life in an unusual intracellular …

… that there was almost nothing. Now, this is back in the 1970s. So, I thought to myself, maybe I can use my … convince those people of that fact, however, a long time, 10 years! ALISON TAKEMURA: Wow. It’s all happened so fast. I … course using modern phylogenetic tools measuring genes, the 16S gene, and comparing it with everything that’s known. So …

… people what that number is. BETSY PARKINSON: So micromolar, 10 to the negative 6th. Nanomolar, 10 to the negative 9th. Picomolar, 10 to the negative 12th. DAN: Yeah. So very-- BETSY PARKINSON: Very, very-- … them back to us and we'll do a PCR analysis to sequence the 16S and things like that to figure out what they actually …

And you have these different types of particulate like 2.5, 10 PPMs and just the size of the particle and how far they … there a way to do that? DAN UDWARY: Well, so there’s good news and bad news on that. So one of the things that we’re … happening. And so we should have somewhere around 10, 11, 12 million BGCs in the database on the release, and that’s …

… as well as a unique opportunity to complete a California K-12 science or math teaching credential as an undergraduate …

So they’re in the picomolar range. ALISON: Picomole… is [10 to the] minus twelve, right? BEN: Yeah, yeah. Well, we are at [10 to the] minus 12 [Molar concentration]- is sufficient to kill cells. So … So I wanted to ask you also about – because you were in the news recently – TSRI has acquired the Pfizer strain …

… welcome back to Natural Prodcast, and this will be episode 11. This continues our little sprint on genome mining. In … tell a quick story. Looking back, the year was probably 2010, 2011, I was a, uh- actually, I’ve got the t-shirt on … microbiome field, many people had just been looking at like 16S taxonomic profiles of these communities looking at who …