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… and the JGI user community below for DNA preparation tools, high molecular-weight DNA extraction and RNA preparation.  … can be found here . DNA Extraction Protocols: … Tips for High Molecular Weight DNA extraction: … RNA Extraction …

… protocols Obtaining DNA and RNA of suitable quantity and quality has been the rate-limiting step for many projects at … collection and storage (from PacBio) Best practices for High Molecular Weight DNA extraction and quality control … Project Manager (PM) a list of organism names (or for metagenomes, sample names).  Need help naming your organisms? …

… by environmental change, focusing on plant resilience — highlighting the vulnerabilities of monocultures and the … and environmental sustainability. … Plant Flagship Genomes are selected through discussion with the JGI Plant … Genomes (OGG) project seeks to develop comparative high quality genomes and annotations across the plant kingdom to …

… using long-read sequencing. Exact library types and quality of finished product depend on genome. Selected … For counting: text file of gene counts (mapped against reference transcriptome or de novo assembly), DGE, alignment … ( IMG ) 280/298 Microbial Improved Draft, Isolate High quality draft assembly of long-read sequence data, …

… data and serving them to users via the Integrated Microbial Genomes & Microbiomes (IMG/M) system. Research Team    … The Integrated Microbial Genomes group is focused on:  Developing state-of-the-art … extracted from metagenomes, and also as a metric for quality control within IMG. While ANI is integrated within …

… and bioeconomy applications. The program provides high-quality, large-scale genomic data to advance microbial … Expansion of sequence space:  The JGI generates reference genomes from diverse bacterial and archaeal …

… terrestrial biogeochemical processes, development of high performance plants and microbes, and understanding … ENVnet can be used in your custom Python applications but highly recommend using the workflow in  GNPS2 .   … In …

I got the chance to do genome mining on more than 50,000 genomes that we derived from meta-genome sequences. As … from the GEM catalog (in green) and previously existing reference genomes (in gray). Around the phylogenetic tree, … but in subtle ways that affect fitness. And so, doing a high level kind of analysis, you just might not be able to …

… scientific discovery at the DOE Office of Science through high performance computing (HPC) and data analysis.  The DOE … To jointly support these missions, and to expand upon highly successful past collaborations, the DOE JGI, NERSC … such factors as genome size, polymorphism level, sample quality and availability, etc.   During scientific peer …

… technology in order to enhance the quality of single-cell genomes and directly link functional information with … DNA amplification, and sequence analysis to recover the genomes of uncultivated microbes. We also apply labeling …

Program uses advanced sequencing technologies to decode the genomes of microbes in their natural environments. This work … Earth’s ecosystems. … The Metagenome Program focuses on the high-throughput sequencing of microbial communities to … catalog of viral, microbial, and microeukaryote genomes and mobile genetic elements, linked to custom …

… similarity to a gene cluster with a known compound from the reference database: It must be identical. It must be that … least a certain level of similarity. So if similarity was higher than a certain kind of volume. And if you do that, … and good assembly. They were able to assemble a really high quality metagenome of the endosphere microbial communities …