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… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … today, run full-fledged marine chemical ecology programs. Two of the great students are in Georgia, in Atlanta, at … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

Moving forward, I’m hoping to get a new one out every two weeks. This time, we’ve got a great interview with Ben … the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very …

Not one of our normal interviews, but instead it’s two interviews in one. My new co-host, Jackie Winter, has … is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … Sequencing Program, and they worked with us to sequence two genomes. Those were of Salinospora tropica and arenicola … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against …

… so they’re secondary metabolite peptides. And there are two ways that nature does this. The more well-known … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… about Antarctica, and we want to talk about molecular networking, because I really enjoyed the review that you wrote … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … lots of stuff. On average, we had something– I think we had two biosynthetic clusters per MAG, which is pretty good …

… the product and deliverables as well as target cycle times from sample receipt to completion of standard analysis.  Raw … sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs …

… in particular to study inter-organismal interactions. Novel approaches are being explored to predict microbial … resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from diverse bacterial and archaeal lineages to improve …

… projects will provide pilot data to assess feasibility of a novel approach or form the foundation for a large-scale CSP proposal submission. Sequence requests should not exceed 3 Tb in total. Proposals … any time as brief white papers. Applicants may submit up to two proposals per year (but may be listed as co-PI on an …