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Sequencing Submission Requirements for DNA Synthesis
When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …
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Sequencing Technologies
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Natural Prodcast Episode 2: The Primer, Part 2
DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have … going to set in. The more you use an antibiotic, the more a bacterium is exposed to it, the greater the chance that that …
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Natural Prodcast Episode 12: Nadine Ziemert
… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger … itself changes a lot. Because you have to be kind of resistant against that one antibiotic that is there. …
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Natural Prodcast Episode 4: Nancy Keller
… out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk … natural products and fungi are induced by growing beside a bacterium and I’m sure the opposite way around, because …
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Natural Prodcast Episode 16: Marcy Balunas
… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … in. And there is quite a bit of sun and exposure to solar radiation with refraction through the ice.  DAN: What’s your … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …
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Products
… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …
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Natural Prodcast Episode 6: Marc Chevrette
… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … strange to me, but I’m new, so… DAN: Well, you know, every bacterium that’s killing another bacterium is an ecological …
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How to Submit Sequencing Samples
… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …
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Microbial Program
… resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … of microbial communities based on their metagenome sequence, in the context of reference isolate genomes …
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Natural Prodcast Episode 13: Francisco Barona Goméz
… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … think the terms we should be using for what we do are, and why there and why it’s important to make those distinctions. … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …
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