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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… about microbiology and natural products from the Great Salt Lake, and you’re going to learn, like me, what a “haboob” … is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a …

… libraries for sequencing. They apply Lean Six Sigma practices to process optimization and development approaches, … The team works hand in hand with the Project Management Office to help ensure the JGI’s genomic capabilities are ready …

… for sample collection and storage (from PacBio) Best practices for High Molecular Weight DNA extraction and quality … holidays). We recommend shipping your samples on dry ice.  Please contact your project manager for alternate … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger … soil. But we also have, in collaboration with other people, lake sediments. We have microbiomes from different …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … NARAYAN: I like to think every enzyme is a little snowflake. JACKIE WINTER: To treat it so it’s a delicate … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh …

… changed the audio editing suite I use which had some nice magic that clean things up. So I didn’t get to talk to … least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

Awesome. Yeah, I’ve heard a lot of good things about Ben. Nice to meet you, Ben. BEN: Hey, yeah! Great, I’m very happy … the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … to less than 5kb in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs …

Program aims to generate large datasets to explore sequence-function relationships, advancing the discovery of … Institute (JBEI), we are developing tools including ICE and DIVA to rapidly design combinatorial construct … Science Group supports researchers exploring how to convert sequence into functional assessments. The team has a …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … to be big pieces somewhere, right? The National Park Service has a great writeup on the kind of gold mining Dan is … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… as well as different types of environments (e.g. soil, lake).  A wide range of metabolites are identified using …