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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … Program leads to discuss available options. Note: In 2021, we conducted an extensive internal product audit to … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs …

Weight DNA extraction and quality control (from PacBio) 2) Review guidelines detailing quantity and quality … must also include a completed Export Certification Form . 2) Obtain Approval for Shipping Before approval to ship is … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

Berkeley National Laboratory. The JGI consists of around 280 researchers and support staff in the Integrative …

… … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …

… secondary metabolism. Welcome back. This will be episode 12 of Natural Prodcast. And it’s our conversation with Nadine … there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … has to do with the mechanism. And so these enzymes use O2, and one of those oxygen atoms gets incorporated into our …

… of this is based on a really great paper that came out in 2020 where this is all explained in detail, so if you’d like … least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … found in Yu et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 1253–1262, 70:3 ALISON TAKEMURA: And so did you … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …