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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… research that drives sustainable solutions in energy, agriculture and ecosystem management. … The Microbial Program … and genome sequencing of underexplored bacterial and archaeal lineages that are important for bioeconomy … resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from …

… the sequencing and comparative analysis of thousands of archaeal and bacterial type strains ( GEBA-type strains … al. (2017) Protein structure determination using metagenome sequence data. Science   355(6322):294-298 Paez-Espino D. et al. (2017) IMG/VR: a database of cultured and uncultured DNA Viruses and retroviruses.  …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … The GEM catalog expands the bacterial and archaeal orders as seen on the phylogenetic tree, with new …

… on the discovery and characterization of novel bacterial, archaeal and eukaryotic microbes and viruses in environmental sequence data. We use multi-omics (metagenomics, …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … annotation ( IMG/M ), mapping 273/273 Metagenome Cell Enrichments Obtained by physical separation of a …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … go see colonies of bacteria just growing? They’re not monocultures there. They’re definitely consortia. There’s layer …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … molecule of water. DAN UDWARY: Mhm. So oxygenases, they’re oxidizing other molecules. ALISON NARAYAN: Correct. Correct. …

… individual tubules.  And so these tubules are like tiny monocultures but the whole of the organ which is about the size … does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … or you know, you’ve run into challenges that demonstrate why this is so important. BEN:  Yeah. So I wouldn’t call it …

… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …