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… metagenomics and metatranscriptomics provide invaluable information on virus diversity and activity in microbial … resources. The main focus of these resource development efforts currently include:  in silico host prediction for uncultivated phages improved annotation and functional …

… through end-to-end automation — and further advancing sequence applications, single cell genomics, biodesign, and … [QA/QC]), large-scale liquid handling automation, and sequence analysis. … An assay used for profiling transcription factor binding sites across … samples in 96-well plates on automated liquid handler platforms, substantially increasing efficiency and scalability. …

… When submitting sequences to the JGI DNA Synthesis program, please include … Sequence files for all constructs should be emailed to  Miranda … File There are several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… to explore the vast and diverse world of microbes. Studying bacteria and archaea, including those associated with … inter-organismal interactions. These insights are crucial for addressing key environmental challenges, such as … to explore microbial diversity — with an emphasis on bacteria and archaea, and including those associated with …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … they spread, why do they spread in a certain way from one bacteria to another, how do they change then, and why are … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… about something I can kind of see like the squid and the bacteria and how the chemistry is going back and forth … does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … into the secondary metabolism of some more novel marine bacteria. You know, I first met Bill as a postdoc working … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

… work like ActDES, which is a curated database of actinobacteria for evolutionary studies, and hopefully we can … least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… of the process needed to prepare and ship samples for sequencing by the JGI.  Additionally it includes … quantity and quality has been the rate-limiting step for many projects at the JGI. The quality of the starting … sequencing workflows and provide guidance as to how to perform sample QC prior to shipment: Importance of Sample QC …

… to completion of standard analysis.  Raw data is provided for all sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … to the  MassIVE repository . Not all products are available for all proposal calls.   Please review the call language …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … find, things that are of interest. And usually, in terms of bacteria and secondary metabolism, those things that we want … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… And a lot of us moved on to work in simpler systems, like bacteria, where we figure we can make more progress faster, … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …