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… and eukaryotic microbes and viruses in environmental sequence data. We use multi-omics (metagenomics, metatranscriptomics, single cell genomics and phylogenomics) and machine learning to identify new divergent lineages and expand the Tree of Life. We then investigate …

… recent work like ActDES, which is a curated database of actinobacteria for evolutionary studies, and hopefully we … least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… a couple biology classes in undergrad, but my PhD was an actinobacteria. And I tell all my students this now, but … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  …

… and state-of-the-art next-generation sequencing to enable biological discovery by the JGI and external DOE … the latest  sequencing technologies has allowed the JGI to increase sequencing output while significantly lowering … for sequencing. They apply Lean Six Sigma practices to process optimization and development approaches, testing …

So we do a couple of different things. Most of them are actinobacteria. And so we get a lot of them from databases … The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain …

Below is a brief description of the process needed to prepare and ship samples for sequencing by the JGI. … permission) demonstrate the importance of proper nucleic acid quantification in our Illumina and PacBio sequencing … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to … take one of those and co-culture them with say one of our Actinobacteria what will happen? Will it create chemistry …

… cutting-edge sequencing, annotation and analysis techniques to explore the vast and diverse world of microbes. Studying … genome sequencing of underexplored bacterial and archaeal lineages that are important for bioeconomy applications. By … resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from …

DAN UDWARY: You’re listening to the Department of Energy Joint Genome Institute’s Natural … more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … a laboratory. Initially, my group really focused on marine actinobacteria. Then we moved into marine proteobacteria. …