Search

When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … we would end up with dead enzymes. So often, when you start monkeying around with your active site– DAN UDWARY: Sure. …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … Program leads to discuss available options. Note: In 2021, we conducted an extensive internal product audit to … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs …

… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … I have been in – working in secondary metabolism for almost 20 years now. Which when I did the math, that was a surprise … environment or produce themselves. It’s the pigments in flowers. It’s communication molecules that some organisms …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … found in Yu et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 1253–1262, 70:3 ALISON TAKEMURA: And so did you … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? … or fat. ALISON TAKEMURA: Is the “enfleurage,” is that “in flower”? What does that mean? CASSANDRA QUAVE: Also “in …

Weight DNA extraction and quality control (from PacBio) 2) Review guidelines detailing quantity and quality … must also include a completed Export Certification Form . 2) Obtain Approval for Shipping Before approval to ship is … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

Berkeley National Laboratory. The JGI consists of around 280 researchers and support staff in the Integrative …

… secondary metabolism. Welcome back. This will be episode 12 of Natural Prodcast. And it’s our conversation with Nadine … there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … we swabbed this stalactite. We used to swab that gypsum flower, that popcorn. JACKIE WINTER: And when you bring back …