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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … MARCY : It’s getting bacteria from there. We work on a fungus garden ant as well as the American honeybee. And so I … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more … susceptible to a fungal infection of rye grain – a specific fungus, Claviceps purpurea, can infect grain. And when it …

Cochliobulis heterostrophis . This is a very well known fungus because it produces a secondary metabolite or natural … out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … to different species.  DAN: Yeah, yeah.  BEN: And then for fungus, even more. And of the fungus, we have more than 2600 …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … same kind of way, where we had– aflatoxin is produced by a fungus called Aspergillus . We were mostly working in … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … DAN UDWARY: That’s weird. I hadn’t heard that before. Why is that? Why would that be the case? ALISON NARAYAN: …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … that will actually protect the fungal farms from other fungus that want to take advantage of that resource. So in … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … the lab of Cameron Currie. They work primarily within the “fungus growing ant” system where there is a symbiont, a …