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… Cas9 needs guide RNA to lead it to the correct sequence to snip — but not all guides are effective. To … approach: CRISPR-Cas9 relies on RNA with a short guiding sequence (20 bp) that directs the endonuclease where to cut, … , the scientists thus set out to identify which sgRNA sequences were reliable. In an elegant, quantitative screen, …

… CheckV can help researchers assess virus sequence fragments and data quality. The Science Through … and computational approaches, more and more virus sequences are being recovered and identified from … the quality and completeness of metagenome-assembled virus sequences vary widely. In a previous effort, an …

… The Impact Despite great strides in generating genome sequences for members of the microbial world through bulk … microbes within metagenome samples and associate their sequences with their roles within their environments. … the complex community and then separating out the sequences representing microbes of interest. When the …

… Streptomyces is more than just a funny word. The 500 species described within the group are responsible for …

… development, dozens of reference genomes and thousands of resequenced sorghum varieties, a resource for breeders and … high-quality reference genomes. By cataloguing a genome sequence for a line of sorghum, reference genomes have … Now, researchers have brought many reference genomes and sequences together at once, producing a pangenome resource …

… domesticate some 60 strains, inserting into them a genetic sequence that acts as a “landing pad.” Mouncey adds, “Once … different gene deletions in four gammaproteobacterial species, and then removing the editing machinery afterward …

… and eukaryotic microbes and viruses in environmental sequence data. Dr. Schulz employs multi-omics (metagenomics, …